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How do you optimise peak separation in HPLC?
we can optimize peak separation by optimizing the HPLC conditions for standard solutions.
Is acid and base hydrophobic or hydrophobic?
hydrophilic and elutes first on HPLC due to high hydrophilicity
What is single point threshold in peak purity of hplc?
Single point threshold is show only peak maxima spectra whenever peak purity index is shows the front, tail and peak maxima spectra. for pure peak we have to consider three point peak purity which shows that your purity angle should be less than purity threshold which is clearly shows that your peak is specrally pure and homogeneous.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
How do you optimise peak separation in HPLC?
we can optimize peak separation by optimizing the HPLC conditions for standard solutions.
Peak-to-valley ratio in HPLC?
to calculate signal to noise use this formulae peak hight *2/noise level
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
How will calculate rrf in hplc?
RRF= (area or height peak X amount IS)/(area or height X amount pk) IS is the internal standard
Is acid and base hydrophobic or hydrophobic?
hydrophilic and elutes first on HPLC due to high hydrophilicity
What is single point threshold in peak purity of hplc?
Single point threshold is show only peak maxima spectra whenever peak purity index is shows the front, tail and peak maxima spectra. for pure peak we have to consider three point peak purity which shows that your purity angle should be less than purity threshold which is clearly shows that your peak is specrally pure and homogeneous.
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
Is the air pressure greater at the peak of the mountain or the base?
At the base.
What is the opposite of the base of a mountain?
the peak of the mountain
What is From the base of Everest toward the peak?
Uphill
