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Single point threshold is show only peak maxima spectra whenever peak purity index is shows the front, tail and peak maxima spectra. for pure peak we have to consider three point peak purity which shows that your purity angle should be less than purity threshold which is clearly shows that your peak is specrally pure and homogeneous.
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How do you calculate hplc assay and hplc purity?
HPLC purity :It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)HPLC assay :It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.HPLC potency :It is measurement of our analyte how potent it is.i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etcFor example:If we have a analyte of some X of purity 99.5%.Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.For 20% solution you will get 99.5% purity and 20% assay.For 60% solution you will get 99.5% purity and 60% assayFor 90% solution you will get 99.5% purity and 90% assay.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
How do you calculate hplc assay and hplc purity?
HPLC purity :It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)HPLC assay :It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.HPLC potency :It is measurement of our analyte how potent it is.i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etcFor example:If we have a analyte of some X of purity 99.5%.Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.For 20% solution you will get 99.5% purity and 20% assay.For 60% solution you will get 99.5% purity and 60% assayFor 90% solution you will get 99.5% purity and 90% assay.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
What analytic method is more preferrable betwee GC and HPLC?
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
Where can one purchase HPLC detectors?
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
