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HPLC purity :
It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.
i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)
HPLC assay :
It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.
HPLC potency :
It is measurement of our analyte how potent it is.
i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etc
For example:
If we have a analyte of some X of purity 99.5%.
Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.
For 20% solution you will get 99.5% purity and 20% assay.
For 60% solution you will get 99.5% purity and 60% assay
For 90% solution you will get 99.5% purity and 90% assay.
What else can I help you with?
What is assay by HPLC?
Assay by HPLC refers to using high-performance liquid chromatography (HPLC) as a technique to quantify the presence and concentration of a specific compound or analyte in a sample. HPLC separates and analyzes components within a mixture based on their interactions with the mobile and stationary phases, allowing for accurate measurement of analyte concentrations. It is commonly used in pharmaceutical, environmental, and food industries for quality control purposes.
Definition of standard in HPLC?
In HPLC, a standard is a known compound with a defined chemical structure and purity used for comparison and identification purposes. Standards are essential for calibrating instruments, determining retention times, and quantifying unknown compounds in samples during analysis.
How do you calculate concentration from peak area in HPLC analysis?
To calculate concentration from peak area in HPLC analysis, you can use the formula: Concentration Peak Area / (Slope x Injection Volume). The peak area is obtained from the chromatogram, the slope is the calibration curve slope, and the injection volume is the volume of the sample injected into the HPLC system.
What does an assay card tell about gold bars?
An assay card provides important information about a gold bar, such as its weight, purity, serial number, and the mint or refinery where it was produced. This helps to verify the authenticity and quality of the gold bar.
What is the difference between potency and assay?
Potency is the purity of the compound ;this states % available of the main component without water and residual solvents Assay is defined as Potency plus water and residual solvents and other remaining fractions please note assay can also be expressed as as is basis or dry basis Assay and Potency are same if assay is exprtessed as as is basis please note Potency word is mainly used for Antibiotics and bilogicals courtsey ; CDER , USFDA ; Dr.Uttam Krishna
What is the formula to calculate assay?
The formula to calculate assay is: Assay (%) = (Actual quantity of substance / Theoretical quantity of substance) x 100. This formula compares the actual quantity of a substance present in a sample to the theoretical quantity that should be present, expressed as a percentage.
What is assay by HPLC?
Assay by HPLC refers to using high-performance liquid chromatography (HPLC) as a technique to quantify the presence and concentration of a specific compound or analyte in a sample. HPLC separates and analyzes components within a mixture based on their interactions with the mobile and stationary phases, allowing for accurate measurement of analyte concentrations. It is commonly used in pharmaceutical, environmental, and food industries for quality control purposes.
How you calculate rrf value in hplc methods?
Yes.
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
Why assay by hplc limit is 98 percent to 102 percent?
If the substance under test is purer than the standard, the assay comes more than 100 pct.
How do you calculate the assay percent in chemicals?
4.4 g of NaOH
Definition of standard in HPLC?
In HPLC, a standard is a known compound with a defined chemical structure and purity used for comparison and identification purposes. Standards are essential for calibrating instruments, determining retention times, and quantifying unknown compounds in samples during analysis.
How do you calculate concentration from peak area in HPLC analysis?
To calculate concentration from peak area in HPLC analysis, you can use the formula: Concentration Peak Area / (Slope x Injection Volume). The peak area is obtained from the chromatogram, the slope is the calibration curve slope, and the injection volume is the volume of the sample injected into the HPLC system.
What does an assay card tell about gold bars?
An assay card provides important information about a gold bar, such as its weight, purity, serial number, and the mint or refinery where it was produced. This helps to verify the authenticity and quality of the gold bar.
Where is an active assay office located?
An active assay office can be found in various countries, typically affiliated with the national mint or a government agency responsible for certifying the purity of precious metals, such as gold or silver. Double-check with the specific country or region in question for the exact location of an active assay office.
What is an assayers office?
Assay offices are institutions set up to assay (test the purity of) items, to protect consumers. Upon successful completion of the assay, (i.e. the metallurgical content is found to be equal or better than that claimed by the maker and it otherwise conforms to the prevailing law) the assay offices typically stamp a hallmark, punze (G.) or poinçon (F.) on the precious metal item to certify its metallurgical content
What is the difference between potency and assay?
Potency is the purity of the compound ;this states % available of the main component without water and residual solvents Assay is defined as Potency plus water and residual solvents and other remaining fractions please note assay can also be expressed as as is basis or dry basis Assay and Potency are same if assay is exprtessed as as is basis please note Potency word is mainly used for Antibiotics and bilogicals courtsey ; CDER , USFDA ; Dr.Uttam Krishna
