HPLC is high-performance liquid chromatography. This method is used for the separation and production of chemicals but also for chemical analysis.
HPLC purity :It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)HPLC assay :It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.HPLC potency :It is measurement of our analyte how potent it is.i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etcFor example:If we have a analyte of some X of purity 99.5%.Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.For 20% solution you will get 99.5% purity and 20% assay.For 60% solution you will get 99.5% purity and 60% assayFor 90% solution you will get 99.5% purity and 90% assay.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Normal phase HPLC separates compounds based on their polarity, with the stationary phase being polar and the mobile phase being nonpolar. Reverse phase HPLC separates compounds based on their hydrophobicity, with the stationary phase being nonpolar and the mobile phase being polar. Normal phase HPLC is typically used for separating polar compounds, while reverse phase HPLC is used for separating nonpolar compounds.
HPLC (High-performance liquid chromatography) is generally considered more advanced than GC (Gas chromatography) due to its broader application range, higher sensitivity, and ability to separate a wider range of compounds. HPLC is often preferred for analyzing complex mixtures and compounds that are not volatile.
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
HPLC purity :It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)HPLC assay :It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.HPLC potency :It is measurement of our analyte how potent it is.i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etcFor example:If we have a analyte of some X of purity 99.5%.Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.For 20% solution you will get 99.5% purity and 20% assay.For 60% solution you will get 99.5% purity and 60% assayFor 90% solution you will get 99.5% purity and 90% assay.
If the substance under test is purer than the standard, the assay comes more than 100 pct.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
why RT was shifting & how to RT calculation in HPLC
One common assay for meprobamate is high-performance liquid chromatography (HPLC), which separates and quantifies the compound based on its unique characteristics. Another method is gas chromatography-mass spectrometry (GC-MS), which can also detect and identify meprobamate in a sample by analyzing its molecular structure and mass. Both assays are sensitive and commonly used in toxicology and clinical settings to measure meprobamate levels in biological samples.
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
mixture of enantiomers can be separated by HPLC
"RS-HPLC method" means "Related Substance HPLC Method".
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
No, assay by mass balance and assay by as is basis are not equivalent. Assay by mass balance calculates the amount of a component based on material balance equations, while assay by as is basis measures the amount of a component without accounting for any changes or losses that may occur during processing.
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.