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What is the deference between GC and GCHS?
GC typically stands for "Guidance Counselor," referring to a professional who provides students with academic, career, and personal support. GCHS typically stands for "Guidance Counselor in a High School," specifying that the counselor works within a high school setting.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
The principle difference between the hplc and gas chromatography?
Thin Layer Chromatography is able to give you information based on thevisible attribute of the separated component. This information has limitations due to the mechanisms by which it is obtained. Typically the TLC plate or TLC paper is stained in order to highlight the components or observed under UV light. Visual attribute include relative spot size, intensity, and permeation distance. Results must be measured manually and often differ from analyst to analyst. High Performance Liquid Chromatographymeasures the visible or UV absorption of the separated components. Advanced equipment using a photo diode array detector can collect absorption data at different wavelengths which is vital in determining peak purity and impurity separation. Using HPLC component data can easily be quantified manually or automatically using software coupled to the instrument. This allows for much more accurate and reproducible resultswith a degree of precision that can not be achieved using TLC. Lastly, HPLC is very fast. The main draw back is the price of instrumentation, software, and maintenance.
What are the key differences between normal phase HPLC and reverse phase HPLC in terms of their separation mechanisms and applications?
Normal phase HPLC separates compounds based on their polarity, with the stationary phase being polar and the mobile phase being nonpolar. Reverse phase HPLC separates compounds based on their hydrophobicity, with the stationary phase being nonpolar and the mobile phase being polar. Normal phase HPLC is typically used for separating polar compounds, while reverse phase HPLC is used for separating nonpolar compounds.
How do you calculate hplc assay and hplc purity?
HPLC purity :It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)HPLC assay :It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.HPLC potency :It is measurement of our analyte how potent it is.i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etcFor example:If we have a analyte of some X of purity 99.5%.Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.For 20% solution you will get 99.5% purity and 20% assay.For 60% solution you will get 99.5% purity and 60% assayFor 90% solution you will get 99.5% purity and 90% assay.
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
How does HPLC complement GC in the analysis environmental products?
i have no answer for it...think yurself...
What analytic method is more preferrable betwee GC and HPLC?
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
What is the deference between GC and GCHS?
GC typically stands for "Guidance Counselor," referring to a professional who provides students with academic, career, and personal support. GCHS typically stands for "Guidance Counselor in a High School," specifying that the counselor works within a high school setting.
What is Cut time in HPLC or GC?
Cut time in HPLC or GC refers to the duration of time set for collecting a specific part of the chromatographic peak. It is typically used when only a particular portion of the peak is of interest for further analysis, allowing for precise collection of that specific component. Cut time ensures efficient sample separation and accurate quantification of the targeted analyte.
Can GC and HPLC instruments be used in same room?
Yes, GC (Gas Chromatography) and HPLC (High Performance Liquid Chromatography) instruments can be used in the same room, as long as certain precautions are taken to prevent interference between the two instruments. This includes proper ventilation to avoid cross-contamination, proper spacing between the instruments to prevent vibrations or electromagnetic interference, and ensuring that the room meets the specific requirements for each instrument.
Where can one purchase HPLC detectors?
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
What is difference between gc and hplc?
gas chromatographt (GC) and High Performance Liquid Chromatography (HPLC) are different , and to understand why you must think about what chromatography is: Chromatography in its simplest form is like putting ink on blotting paper and watching the colours separate. Liquid chromatoraphy uses a "column" which is made from bare or bonded silica, it separates a mixture of compounds by how polar they are. You can use a gradient of different solvents. GC also uses a column, but it is a capillary column and instead of using a liquid to carry your mixture which needs to be separated it uses a carrier gas, like nitrogen. You can vary the temperatures in both LC and GC to aid better resolution. GC is used for more volatile compounds and LC is used more less volatile. HPLC usually refers to reversed phase, normal phase is where the column is vare silica which is very polar. Bonded silica is bonded with hydrocarbons which is non polar. The thing to remember is that "like attracts like" so if the column in non polar, the compound to elute first will be the most polar. To summarise, they are both separation techniques, one uses gas and the other liquid. You would choose which one to uese depending on how volatile the compounds which you want to separate are. Vishal Bobade NCL,Pune
What does medical code 82542 stand for?
CPT Code 82542- Column chromatography/mass spectrometry (eg, GC/MS, or HPLC/MS), analyte not elsewhere specified; quantitative, single stationary and mobile phase.
What can one read in the HPLC Column?
In an HPLC column one can see very small molecules such as ATP, histidine, glucose, uracil, and pyridine. It is a form high quality of liquid Chromatography.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
