i have no answer for it...think yurself...
yes
"RS-HPLC method" means "Related Substance HPLC Method".
HPLC is high-performance liquid chromatography. This method is used for the separation and production of chemicals but also for chemical analysis.
how long time gradient reach column.
yes
George Lunn has written: 'HPLC Methods for Pharmaceutical Analysis' -- subject(s): Analysis, Chemistry, Pharmaceutical, Chromatography, High Pressure Liquid, Drugs, High Pressure Liquid Chromatography, High performance liquid chromatography, Methods, Pharmaceutical chemistry 'HPLC Methods for Pharmaceutical Analysis, Volumes 2-4 (HPLC Methods for Pharmaceutical Analysis)' 'Destruction of hazardous chemicals in the laboratory' -- subject(s): Safety measures, Hazardous wastes, Chemical laboratories 'HPLC Methods for Pharmaceutical Analysis VOLUME 2 A-D'
Edward F. Rossomando has written: 'HPLC in enzymatic analysis' -- subject(s): Enzymatic analysis, High performance liquid chromatography
This instrument plays an important part in analysis of materials. Without a good reproducibility the machine would be of little value.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
HPLC is advanced
Most often it doesn't. Are you thinking about gas chromatography? I have used HPLC at room temperature for reversed phase analysis of organics and I have used it at 4 degrees C for protein purification. Only once have I done any at elevated temperature. Gas chromatography uses gas-liquid partitioning though and is almost always done at higher temperatures.
why RT was shifting & how to RT calculation in HPLC