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Most often it doesn't. Are you thinking about gas chromatography?
I have used HPLC at room temperature for reversed phase analysis of organics and I have used it at 4 degrees C for protein purification. Only once have I done any at elevated temperature.
Gas chromatography uses gas-liquid partitioning though and is almost always done at higher temperatures.
What else can I help you with?
Why sample need to be heated before heating in muffle furnace?
Heating a sample before placing it in a muffle furnace helps to remove any moisture or volatile impurities that could affect the results of the heating process. This pre-heating step ensures that the sample is dry and clean, allowing for more accurate and consistent results when it is heated in the muffle furnace.
What are the disadvantages of instrumental analysis?
Can get expensiveNeeds certain special skills and lots of training to be able to do itIt can only give results which can be compared to those we already haveHope this helps a bit :)
What is HPLC normally used to detect?
HPLC (High Performance Liquid Chromatography) is typically used to detect and quantify compounds in a mixture. It is commonly used in analytical chemistry to separate, identify, and quantify components in complex mixtures such as pharmaceuticals, chemicals, food, and environmental samples.
Will heating well water show iron bacteria?
Not necessarily. You will see iron bacteria in the pipes or first filter as an orange jello-like substance. -Take a sample for analysis to be sure, you may need filters.Not necessarily. You will see iron bacteria in the pipes or first filter as an orange jello-like substance. -Take a sample for analysis to be sure, you may need filters.
How fast do you heat the sample when determining a melting point?
The apparatus used for determining the melting point will heat the sample at an appropriate rate. All you need to do is watch and note the point at which the sample melts, as should be demonstrated to you by your lab supervisor.
How fresh does a cat stool sample need to be for accurate testing and analysis?
A cat stool sample should be fresh, ideally collected within 24 hours, for accurate testing and analysis.
What causes bad odor in well water?
i would get it tested there might be sulpher in it or it may be polluted
Why sample need to be diluted?
Samples sometimes need to be diluted to bring their concentration within the range of the measurement method, to prevent interference or saturation of the detector, or to ensure that the sample is compatible with the analytical equipment being used. Dilution can also help reduce matrix effects and improve the accuracy of the analysis.
Why sample need to be heated before heating in muffle furnace?
Heating a sample before placing it in a muffle furnace helps to remove any moisture or volatile impurities that could affect the results of the heating process. This pre-heating step ensures that the sample is dry and clean, allowing for more accurate and consistent results when it is heated in the muffle furnace.
What are the weaknesses of gas chromatography?
Only compounds that are volatile can be analyzed by gas chromatography. Some compounds hence have to be modified so that they are volatile. These compounds also need to be thermally stable as the volume needs to be heated to a high temperature for analysis. In addition, quantitative introduction of the sample is difficult as only small volumes are injected.
Method validation was done in one column now the column has been changed for sample analysis weatherwe need to perform method validation once again or not?
you need equivalency study only by prepare 6 sample in the tow column (they should be same result)
What is the procedure for collecting a stool sample from a cat for veterinary analysis?
To collect a stool sample from a cat for veterinary analysis, you will need to use a clean container and a disposable glove. Wait for the cat to defecate, then use the glove to pick up a small sample of the stool and place it in the container. Make sure to label the container with the cat's name and the date, and refrigerate it if you cannot bring it to the vet immediately.
Does one need to use multilevel analysis when their data set is an unstratified simple random sample?
Good question. The short answer in no. Multistage or cluster sampling create artificial dependencies in the data that need to be taken into account using multilevel or mixed design software. If you sample the data randomly, I think you can do a multilevel analysis using generalized least squares. I have not seen this point made before.
Why does the sample need to be heated over a Bunsen burner before heating in dry ashing?
The scope is to eliminate water, volatile compounds, substances easily thermodegradable before the calcining in a muffle furnace.
What is NIR Analysis?
NIR analysis is a non-destructive analytical technique that uses near-infrared light to determine the chemical composition of a sample. It is commonly used in various industries such as food, agriculture, pharmaceuticals, and environmental monitoring to analyze properties like moisture content, protein levels, and chemical composition. NIR analysis is valued for its speed, accuracy, and ability to provide real-time data without the need for sample preparation.
What is a sample marketing plan?
If you want to know a simple marketing plan you have come to the right place. What you need to do is: Write up an executive summary, a company description, the product or service, the market analysis, the web plan summary, the management team, and the financial analysis.
What are the disadvantages of an internal standard in gas chromatography?
Disadvantages of using an internal standard in gas chromatography include the need for additional sample processing steps, the potential for introducing errors during the mixing of the internal standard with the sample, and the possibility of the internal standard not behaving identically to the target analyte during the analysis.
