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How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Can you use a C18 column for HPLC with fluorescence detector and methanol as the mobile phase?
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
What are the key differences between normal phase HPLC and reverse phase HPLC in terms of their separation mechanisms and applications?
Normal phase HPLC separates compounds based on their polarity, with the stationary phase being polar and the mobile phase being nonpolar. Reverse phase HPLC separates compounds based on their hydrophobicity, with the stationary phase being nonpolar and the mobile phase being polar. Normal phase HPLC is typically used for separating polar compounds, while reverse phase HPLC is used for separating nonpolar compounds.
Definition of standard in HPLC?
In HPLC, a standard is a known compound with a defined chemical structure and purity used for comparison and identification purposes. Standards are essential for calibrating instruments, determining retention times, and quantifying unknown compounds in samples during analysis.
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
Why is anthracene is used for calibration of hplc?
Anthracene is used as a calibration standard in High Performance Liquid Chromatography (HPLC) because it has a well-defined retention time and peaks in the UV-visible spectrum, making it easy to detect and quantify. Its consistent behavior helps in determining retention times, resolving power, and column efficiency during method development and troubleshooting in HPLC.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
How does one apply the HPLC princple?
The HPLC principle is used to separate liquid mixtures into individual parts. It's frequently used in several field such as medicine and law. HPLC is specifically used in sports to test for performance enhancing drugs in urine.
Where can one purchase HPLC detectors?
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
Can you use a C18 column for HPLC with fluorescence detector and methanol as the mobile phase?
Yes, you can use a C18 column and methanol as a mobile phase with fluorescence detector. Fluorescence detector is generally used as it can detect the presence of compounds at a very low concentration.
How would forensic scientist detect codeine?
HPLC (high pressure liquid chromatography), hair samples and bodily fluids can be tested this way.
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
What is dead volume HPLC?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
Which are the top commercial uses for HPLC?
HPLC is used to analyze, identify, purify and quantify organic compounds.
What are the key differences between normal phase HPLC and reverse phase HPLC in terms of their separation mechanisms and applications?
Normal phase HPLC separates compounds based on their polarity, with the stationary phase being polar and the mobile phase being nonpolar. Reverse phase HPLC separates compounds based on their hydrophobicity, with the stationary phase being nonpolar and the mobile phase being polar. Normal phase HPLC is typically used for separating polar compounds, while reverse phase HPLC is used for separating nonpolar compounds.
