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How does an RID detector work for HPLC?
An RID (Refractive Index Detector) for HPLC works by measuring changes in refractive index caused by the presence of analytes eluting from the column. As analytes pass through the detector cell, they displace the mobile phase, causing changes in refractive index that are detected and converted into a signal. The signal is then plotted against retention time to create a chromatogram that can be used to identify and quantify analytes in the sample.
Basic parts of hplc?
(1) Solvent reservoirs, (2) Solvent degasser, (3) Gradient valve, (4) Mixing vessel for delivery of the mobile phase, (5) High-pressure pump, (6) Switching valve in "inject position", (6') Switching valve in "load position", (7) Sample injection loop, (8) Pre-column (guard column), (9) Analytical column, (10) Detector (i.e. IR, UV), (11) Data acquisition, (12) Waste or fraction collector. Please seehttp://en.wikipedia.org/wiki/File:HPLC_apparatus.svg
How can the clarity of a chromatogram be improved?
The clarity of a chromatogram can be improved by optimizing the chromatographic conditions, such as adjusting the mobile phase composition or flow rate, using a column with better resolution, and ensuring proper sample preparation techniques. Additionally, improving the detector sensitivity and reducing background noise can also enhance the clarity of the chromatogram.
How much dmso should be used for a c-18 hplc column to knock out everything retained in the column?
Depends on the column..... For a regular C18, try running 10% methanol through and then full-loop injections of 50% DMSO repeatedly until you're happy with the result. Also, reduce the run-time. For me, 3 minutes for a 2x50mm.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Can C18 column for HPLC be used with UV and visible spectra detector and Acetonitril as mobile phase?
Of course.... there isn´t problem....
What is mean by mobile sniffer?
it is a mobile phone detector
Will a metal detector detect a mobile phone in grass?
Yes the metal detector would but it is a large object and you would see it also.
How does an RID detector work for HPLC?
An RID (Refractive Index Detector) for HPLC works by measuring changes in refractive index caused by the presence of analytes eluting from the column. As analytes pass through the detector cell, they displace the mobile phase, causing changes in refractive index that are detected and converted into a signal. The signal is then plotted against retention time to create a chromatogram that can be used to identify and quantify analytes in the sample.
How you can analyze pregabalin on HPLC with UV detector?
We can quantitatively analyse pregabalin on hplc with uv detector, wavelength will be 210 n.m. and mobile phase will be 5 % acetonitrile. standard & sample solution preparation should be in mobile phase.
What is mobile phase?
The mobile phase as indicated is the moving phase. Either the mobile or stationary phase is polar and the other is Non-polar. A common polar phase is Methanol, and non-polar is hexane
Retention time in Hplc?
Retention time in High Performance Liquid Chromatography (HPLC) refers to the time it takes for a compound to travel through the chromatography column and elute from the detector. It is a key parameter for identifying and characterizing compounds in a sample. Retention time is influenced by factors such as the column type, mobile phase composition, and compound properties.
Basic parts of hplc?
(1) Solvent reservoirs, (2) Solvent degasser, (3) Gradient valve, (4) Mixing vessel for delivery of the mobile phase, (5) High-pressure pump, (6) Switching valve in "inject position", (6') Switching valve in "load position", (7) Sample injection loop, (8) Pre-column (guard column), (9) Analytical column, (10) Detector (i.e. IR, UV), (11) Data acquisition, (12) Waste or fraction collector. Please seehttp://en.wikipedia.org/wiki/File:HPLC_apparatus.svg
What is the principle of GC?
Gas chromatography (GC) separates and analyzes compounds based on their distribution between a stationary phase (inside a column) and a mobile phase (a carrier gas). Compounds with different affinities for the stationary phase will travel through the column at different rates, allowing for their separation. The detector at the end of the column detects these compounds based on their unique retention times.
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
Do people really use a portable gas detector?
Yes people use a portable gas detector. This enables them to move their location and detect gas there. This can be better if you are more mobile and going to different locations.
What will be the mobile phase for Thin Layer Chromatography of Schiff base?
Schiff bases are imines formed by the condensation of aldehydes or ketones with primary amines. The mobile phase for this could be ethyl acetate in hexane or chloroform in methanol.
