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How much dmso should be used for a c-18 hplc column to knock out everything retained in the column?
Wiki User
∙ 15y ago
Depends on the column.....
For a regular C18, try running 10% methanol through and then full-loop injections of 50% DMSO repeatedly until you're happy with the result. Also, reduce the run-time. For me, 3 minutes for a 2x50mm.
Wiki User
∙ 15y ago
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