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why the sample need dilution
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∙ 11y ago
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Will isolated colonies of bacteria always be in the fourth sector on the streak plate?
No, of course it is possible that one could start out with an already diluted sample in the first step. So then perhaps in the third sector some isolated colonies may form, because the amount of bacteria have been diluted enough.
Under what kind of plating technique would you find deep or buried colonies?
In pour plate method, in this method of plating or culturing the microbial sample first diluted and pour in empty plate and after that growth media is poured in it and which is then skake firmly. As the microbial suspension or sample is distributed in media the growth of bacteria occurs in buried of deep positions.
Why is it necessary to use only diluted cultures that contain 100 to 200 cells for successful spread plate?
If this is the question asked in your lab manual, welcome to the club. That's because diluted cultures produce little blue men and some pink powders also lots of chocolates when they are put on a spread plate and it yields a successful happy life for you. Tell that to your teacher and you'll get an AA.
What is the importance of a pour plate in microbiology?
Extensively used with procaryotes and fungi, a pour plate can yield isolate colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain seprate colonies when plating. The pour plate can be used to determine the number of cells in a population.
Why must you use a thin specimen when viewing with a light microscope?
For a light transmission microscope, a thin sample is needed in order for the light to pass through the sample from the light source on the other side. However, you can use a dissection microscope (another type of light microscope) which illuminates the sample from above, eliminating the need for a thin sample size. As the name suggests, this is normally used in dissections, and is of low magnification.
Can a lab read a diluted sample?
no i have personally done this every time i have taken a test and passed. but you can get in trouble for having a diluted sample.
Can diluted urine give a false negative?
If you deliver a diluted sample, they'll flunk you just for the dilution.
Is a diluted urine sample the same as a positive test?
sometimes!
What is a negative diluted urine sample?
A diluted urine drug test is one where the sample was diluted. It happens in two ways--you drank too much water to make yourself pee (DOT's standard says drink no more than 40 ounces of water), or you put water in your sample after you peed it out.A negative diluted test says "no drugs in this sample passed the test thresholds, but it was diluted so there MIGHT be drugs in here." Basically, a diluted negative is a test failure.
Would marijuana show up on a very diluted urine sample?
Nope!
WHY pH increases of strong acid when diluted?
When the acid is is diluted; its pH has a chance increase above seven and nake the sample;solution; Etc acidic.
Will the lab still test a very diluted sample and will cocaine still show up if it is diluted with lots of water?
It really depends on the amount of cocaine that was taken, and the strength of the test. If a lab test probably caught.
Can you combine a clean donors urine with your own diluted sample to pass a drug screen?
only if you are stupid enought to do that and risk jail
How many moles of Cu were in the original sample which had been effectively diluted to a volume of 200 m?
(0.102gx1mole)/99g CuCl = 1.031x10^-3 moles
Will isolated colonies always be in the second sector on the streak plate?
No. It depends on the number of bacteria present in the initial sample. If the number of bacteria in the initial sample are limited, you may get isolated colonies in the first streak. If the number of bacteria in the sample are high, it may take several streaks before the sample is diluted to the point where isolated colonies are evident.
I have been on fed probation for 3 years always complied what will happen if I miss a urine because im out of town he said the last one was diluted I did nothing different New po for last 3 weeks?
Get a lawyer. There is no way to determine in standard practices whether a sample is diluted or not. A full laboratory spectral analysis of the sample would need to be conducted, and this is neither cost effective nor judicially prudent without probable cause. Not to mention with current federal lab delays, it would take months for him to obtain the results.
How to calculate BOD value in a sample?
if unseeded, BOD = (D1-D2)/Pif seeded, BOD = ((D1-D2)-(B1-B2)f)/PD1 = DO of diluted seeded wastewaterD2 = DO of wastewater after incubationB1 = DO of diluted seed sampleB2 = DO of seed sample after incubationf = ratio of seed volume in seeded wastewater test to seed volume in BOD test on seedP = decimal fraction of wastewater sample used. (vol. of wastewater)/(vol. of dilution water plus wastewater)Maybe that helps
