yes
HPLC is high-performance liquid chromatography. This method is used for the separation and production of chemicals but also for chemical analysis.
how long time gradient reach column.
concentration of buffer effects in buffer strength
This instrument plays an important part in analysis of materials. Without a good reproducibility the machine would be of little value.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
"RS-HPLC method" means "Related Substance HPLC Method".
HPLC is high-performance liquid chromatography. This method is used for the separation and production of chemicals but also for chemical analysis.
i have no answer for it...think yurself...
the same guidelines for method validation
Method development is a process amenable to continuous improvement
how long time gradient reach column.
specificity and selectivity
yes
i have two methods in bith methods the column used is c8 one method is pH at 4.0 with triethyl amine and another with ionpair in both casess small amount of THF is required to separate critical impurities for dosage form the suggested method is with ion pair
George Lunn has written: 'HPLC Methods for Pharmaceutical Analysis' -- subject(s): Analysis, Chemistry, Pharmaceutical, Chromatography, High Pressure Liquid, Drugs, High Pressure Liquid Chromatography, High performance liquid chromatography, Methods, Pharmaceutical chemistry 'HPLC Methods for Pharmaceutical Analysis, Volumes 2-4 (HPLC Methods for Pharmaceutical Analysis)' 'Destruction of hazardous chemicals in the laboratory' -- subject(s): Safety measures, Hazardous wastes, Chemical laboratories 'HPLC Methods for Pharmaceutical Analysis VOLUME 2 A-D'
concentration of buffer effects in buffer strength
Edward F. Rossomando has written: 'HPLC in enzymatic analysis' -- subject(s): Enzymatic analysis, High performance liquid chromatography