This instrument plays an important part in analysis of materials. Without a good reproducibility the machine would be of little value.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
HPLC is advanced
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
NO. But, if you can't reproduce it, it ain't science.
depending on pH resolution is there
the reproducibility refers to how close a group of measurements are to each other
Accuract is how accurate you are at somehting and rreproducibility is how reproducibility you are at something.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
HPLC is advanced
Precision.
why RT was shifting & how to RT calculation in HPLC
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
By telling others what you learned keeps science going. Then other scientists can then conduct their own tests based on your resuts.
the ability to be reproduced or copied.