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How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
What are the key differences between normal phase HPLC and reverse phase HPLC in terms of their separation mechanisms and applications?
Normal phase HPLC separates compounds based on their polarity, with the stationary phase being polar and the mobile phase being nonpolar. Reverse phase HPLC separates compounds based on their hydrophobicity, with the stationary phase being nonpolar and the mobile phase being polar. Normal phase HPLC is typically used for separating polar compounds, while reverse phase HPLC is used for separating nonpolar compounds.
Which one is advanced gc or hplc?
HPLC (High-performance liquid chromatography) is generally considered more advanced than GC (Gas chromatography) due to its broader application range, higher sensitivity, and ability to separate a wider range of compounds. HPLC is often preferred for analyzing complex mixtures and compounds that are not volatile.
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
Definition of standard in HPLC?
In HPLC, a standard is a known compound with a defined chemical structure and purity used for comparison and identification purposes. Standards are essential for calibrating instruments, determining retention times, and quantifying unknown compounds in samples during analysis.
Why ordinary liquid pump cannot be used in hplc system?
An ordinary liquid pump cannot be used in HPLC systems because HPLC requires pumps that can generate high pressure to push solvents through the column at a constant and precise flow rate. Ordinary pumps do not provide the level of pressure and flow rate control needed for HPLC separations, which can negatively impact the accuracy and reproducibility of chromatographic results. HPLC pumps are specifically designed to handle the high pressures and flow rates required for efficient separations.
Is hplc chromatography reliable?
Yes, HPLC chromatography is a highly reliable technique for separating and analyzing compounds based on their physical and chemical properties. It is widely used in various industries such as pharmaceuticals, food testing, environmental analysis, and more due to its high sensitivity, accuracy, and reproducibility.
How pH play an important role in hplc sepration?
depending on pH resolution is there
Is it true that only reproducibility is important when making scientific measurements?
NO. But, if you can't reproduce it, it ain't science.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
What does the reproducibility of data refer to?
the reproducibility refers to how close a group of measurements are to each other
What is the difference between reproducibility and accuracy?
Accuract is how accurate you are at somehting and rreproducibility is how reproducibility you are at something.
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What is post run in HPLC?
Post run in HPLC refers to the time after the completion of a chromatographic analysis where the system continues running to ensure that any remaining compounds are fully flushed out of the column and detector to prevent contamination and achieve a clean baseline for subsequent runs. It is an important step to maintain the integrity and performance of the HPLC system.
Is this sentence true or false only reproducibility is important when making scientific measurements?
False. In addition to reproducibility, accuracy, precision, and validity are also important when making scientific measurements. Reproducibility ensures that the results can be consistently obtained by different researchers, while accuracy refers to how closely the measured value aligns with the true value. Precision relates to the consistency and reliability of the measurements, while validity ensures that the measurements are actually measuring what they claim to measure.
Why is it important to establish the reproducibility of data?
Establishing the reproducibility of data is important to ensure the reliability and validity of research findings. It allows other researchers to independently verify and build upon the results, contributing to the overall credibility of the scientific community. Reproducibility also helps identify errors or inconsistencies in the data, promoting transparency and trust in the research process.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
