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What analytic method is more preferrable betwee GC and HPLC?
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
What kind of species can be separated by high performance liquid chromatography but not gas chromatography?
Polar and non-volatile compounds, such as large biomolecules like proteins or carbohydrates, can be separated by high performance liquid chromatography but not gas chromatography due to differences in their chemical properties and interaction with the stationary phase. Gas chromatography is more suitable for separating volatile and non-polar compounds based on their volatility and interaction with the stationary phase.
What is difference between rrlc and hplc?
gas chromatographt (GC) and High Performance Liquid Chromatography (HPLC) are different , and to understand why you must think about what chromatography is: Chromatography in its simplest form is like putting ink on blotting paper and watching the colours separate. Liquid chromatoraphy uses a "column" which is made from bare or bonded silica, it separates a mixture of compounds by how polar they are. You can use a gradient of different solvents. GC also uses a column, but it is a capillary column and instead of using a liquid to carry your mixture which needs to be separated it uses a carrier gas, like nitrogen. You can vary the temperatures in both LC and GC to aid better resolution. GC is used for more volatile compounds and LC is used more less volatile. HPLC usually refers to reversed phase, normal phase is where the column is vare silica which is very polar. Bonded silica is bonded with hydrocarbons which is non polar. The thing to remember is that "like attracts like" so if the column in non polar, the compound to elute first will be the most polar. To summarise, they are both separation techniques, one uses gas and the other liquid. You would choose which one to uese depending on how volatile the compounds which you want to separate are. Vishal Bobade NCL,Pune
Can TLC or paper chromatography be used to separate and identify very volatile substances?
Thin layer chromatography is not a method that can be used for very volatile substances. When a thin layer plate is removed from a developing tank, one needs to evaporate off the developing solvent, which is sometimes done by heating the plate or moving it thorugh a stream of air. Any volatile components on the plate would be removed at the same time. In order to separate and identify volatile compounds, you'd use hplc or gc (high performance liquid or gas chromatography.
What is Cut time in HPLC or GC?
Cut time in HPLC or GC refers to the duration of time set for collecting a specific part of the chromatographic peak. It is typically used when only a particular portion of the peak is of interest for further analysis, allowing for precise collection of that specific component. Cut time ensures efficient sample separation and accurate quantification of the targeted analyte.
Which one is advanced gc or hplc?
HPLC (High-performance liquid chromatography) is generally considered more advanced than GC (Gas chromatography) due to its broader application range, higher sensitivity, and ability to separate a wider range of compounds. HPLC is often preferred for analyzing complex mixtures and compounds that are not volatile.
What analytic method is more preferrable betwee GC and HPLC?
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
GC method of analysis for 6-Bromohexyl trimethyl ammonium bromide?
No salt of organic compounds can be possibly analysed by gc subbu
What is difference between gc and hplc?
gas chromatographt (GC) and High Performance Liquid Chromatography (HPLC) are different , and to understand why you must think about what chromatography is: Chromatography in its simplest form is like putting ink on blotting paper and watching the colours separate. Liquid chromatoraphy uses a "column" which is made from bare or bonded silica, it separates a mixture of compounds by how polar they are. You can use a gradient of different solvents. GC also uses a column, but it is a capillary column and instead of using a liquid to carry your mixture which needs to be separated it uses a carrier gas, like nitrogen. You can vary the temperatures in both LC and GC to aid better resolution. GC is used for more volatile compounds and LC is used more less volatile. HPLC usually refers to reversed phase, normal phase is where the column is vare silica which is very polar. Bonded silica is bonded with hydrocarbons which is non polar. The thing to remember is that "like attracts like" so if the column in non polar, the compound to elute first will be the most polar. To summarise, they are both separation techniques, one uses gas and the other liquid. You would choose which one to uese depending on how volatile the compounds which you want to separate are. Vishal Bobade NCL,Pune
How does HPLC complement GC in the analysis environmental products?
i have no answer for it...think yurself...
What kind of species can be separated by high performance liquid chromatography but not gas chromatography?
Polar and non-volatile compounds, such as large biomolecules like proteins or carbohydrates, can be separated by high performance liquid chromatography but not gas chromatography due to differences in their chemical properties and interaction with the stationary phase. Gas chromatography is more suitable for separating volatile and non-polar compounds based on their volatility and interaction with the stationary phase.
The principle difference between the hplc and gas chromatography?
Thin Layer Chromatography is able to give you information based on thevisible attribute of the separated component. This information has limitations due to the mechanisms by which it is obtained. Typically the TLC plate or TLC paper is stained in order to highlight the components or observed under UV light. Visual attribute include relative spot size, intensity, and permeation distance. Results must be measured manually and often differ from analyst to analyst. High Performance Liquid Chromatographymeasures the visible or UV absorption of the separated components. Advanced equipment using a photo diode array detector can collect absorption data at different wavelengths which is vital in determining peak purity and impurity separation. Using HPLC component data can easily be quantified manually or automatically using software coupled to the instrument. This allows for much more accurate and reproducible resultswith a degree of precision that can not be achieved using TLC. Lastly, HPLC is very fast. The main draw back is the price of instrumentation, software, and maintenance.
What is the principle of GC?
Gas chromatography (GC) separates and analyzes compounds based on their distribution between a stationary phase (inside a column) and a mobile phase (a carrier gas). Compounds with different affinities for the stationary phase will travel through the column at different rates, allowing for their separation. The detector at the end of the column detects these compounds based on their unique retention times.
What is difference between rrlc and hplc?
gas chromatographt (GC) and High Performance Liquid Chromatography (HPLC) are different , and to understand why you must think about what chromatography is: Chromatography in its simplest form is like putting ink on blotting paper and watching the colours separate. Liquid chromatoraphy uses a "column" which is made from bare or bonded silica, it separates a mixture of compounds by how polar they are. You can use a gradient of different solvents. GC also uses a column, but it is a capillary column and instead of using a liquid to carry your mixture which needs to be separated it uses a carrier gas, like nitrogen. You can vary the temperatures in both LC and GC to aid better resolution. GC is used for more volatile compounds and LC is used more less volatile. HPLC usually refers to reversed phase, normal phase is where the column is vare silica which is very polar. Bonded silica is bonded with hydrocarbons which is non polar. The thing to remember is that "like attracts like" so if the column in non polar, the compound to elute first will be the most polar. To summarise, they are both separation techniques, one uses gas and the other liquid. You would choose which one to uese depending on how volatile the compounds which you want to separate are. Vishal Bobade NCL,Pune
Can TLC or paper chromatography be used to separate and identify very volatile substances?
Thin layer chromatography is not a method that can be used for very volatile substances. When a thin layer plate is removed from a developing tank, one needs to evaporate off the developing solvent, which is sometimes done by heating the plate or moving it thorugh a stream of air. Any volatile components on the plate would be removed at the same time. In order to separate and identify volatile compounds, you'd use hplc or gc (high performance liquid or gas chromatography.
Silicone rubber test method?
Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS)At present, GC and GC-MS have become the most commonly used qualitative and quantitative detection methods of silicone rubber for volatile cyclosiloxane in food contact silicone rubber.
What is the deference between GC and GCHS?
GC typically stands for "Guidance Counselor," referring to a professional who provides students with academic, career, and personal support. GCHS typically stands for "Guidance Counselor in a High School," specifying that the counselor works within a high school setting.
