0
gas chromatographt (GC) and High Performance Liquid Chromatography (HPLC) are different , and to understand why you must think about what chromatography is:
Chromatography in its simplest form is like putting ink on blotting paper and watching the colours separate.
Liquid chromatoraphy uses a "column" which is made from bare or bonded silica, it separates a mixture of compounds by how polar they are. You can use a gradient of different solvents.
GC also uses a column, but it is a capillary column and instead of using a liquid to carry your mixture which needs to be separated it uses a carrier gas, like nitrogen. You can vary the temperatures in both LC and GC to aid better resolution.
GC is used for more volatile compounds and LC is used more less volatile. HPLC usually refers to reversed phase, normal phase is where the column is vare silica which is very polar. Bonded silica is bonded with hydrocarbons which is non polar.
The thing to remember is that "like attracts like" so if the column in non polar, the compound to elute first will be the most polar.
To summarise, they are both separation techniques, one uses gas and the other liquid. You would choose which one to uese depending on how volatile the compounds which you want to separate are.
Vishal Bobade NCL,Pune
What else can I help you with?
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
How does HPLC complement GC in the analysis environmental products?
i have no answer for it...think yurself...
Which one is advanced gc or hplc?
HPLC (High-performance liquid chromatography) is generally considered more advanced than GC (Gas chromatography) due to its broader application range, higher sensitivity, and ability to separate a wider range of compounds. HPLC is often preferred for analyzing complex mixtures and compounds that are not volatile.
What is the difference between gc hss and gc als?
What is the difference beween ALS and HSS IN GAS CHROMOTRAGHPY. When to used either of the apparatus
What analytic method is more preferrable betwee GC and HPLC?
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
What is the deference between GC and GCHS?
GC typically stands for "Guidance Counselor," referring to a professional who provides students with academic, career, and personal support. GCHS typically stands for "Guidance Counselor in a High School," specifying that the counselor works within a high school setting.
Difference between lc-ms and gc-ms in pharmaCEUTICAL industry?
no
Can GC and HPLC instruments be used in same room?
Yes, GC (Gas Chromatography) and HPLC (High Performance Liquid Chromatography) instruments can be used in the same room, as long as certain precautions are taken to prevent interference between the two instruments. This includes proper ventilation to avoid cross-contamination, proper spacing between the instruments to prevent vibrations or electromagnetic interference, and ensuring that the room meets the specific requirements for each instrument.
What is Difference Between HPLC UV detector Spectrophotometer uv detector?
HPLC UV detector is a component used in high-performance liquid chromatography (HPLC) to monitor eluent absorbance, while a spectrophotometer UV detector is a standalone instrument used to measure the absorption of light at different wavelengths. HPLC UV detectors are specifically tailored for chromatography applications, whereas spectrophotometer UV detectors are more versatile and used for various analytical purposes.
What is Cut time in HPLC or GC?
Cut time in HPLC or GC refers to the duration of time set for collecting a specific part of the chromatographic peak. It is typically used when only a particular portion of the peak is of interest for further analysis, allowing for precise collection of that specific component. Cut time ensures efficient sample separation and accurate quantification of the targeted analyte.
What are the key differences between reverse phase and normal phase HPLC techniques?
Reverse phase and normal phase HPLC techniques differ primarily in the polarity of the stationary phase and mobile phase. In reverse phase HPLC, the stationary phase is nonpolar and the mobile phase is polar, while in normal phase HPLC, the stationary phase is polar and the mobile phase is nonpolar. This polarity difference affects the retention and separation of compounds in the sample.
What is difference between high pressure liquid chromatography and high performance lequid chromatography?
High pressure liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) are often used interchangeably. HPLC refers to modern liquid chromatography systems with high resolution and efficiency, while high pressure liquid chromatography specifically highlights the use of higher pressures in the system to improve separation and speed. Both terms generally refer to the same chromatographic technique.
