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What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
What is the definition of method development by hplc?
Method development is a process amenable to continuous improvement
What are the HPLC Calibration parameters and elaborate it?
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
What are the columns used in hplc?
* large columns, specifically for packing. * analytical columns, for quantitative analysis, usually accompanied by a UV-vis detector. * Narrow bore columns, for more sensitive analysis * capillary columns, very this silica columns used almost exclusively with GC mass spectroscopy. * packed bed columns. with silica beads. and may have groups attached, e.g. C18.
How you calculate rrf value in hplc methods?
Yes.
How will calculate rrf in hplc?
To calculate relative retention factor (RRF) in HPLC, you need to divide the retention time of the compound of interest by the retention time of the reference compound. The formula is RRF = (Retention time of compound of interest) / (Retention time of reference compound). This value helps in comparison and identification of compounds in the chromatogram.
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
Beta coefficient Given the following information determine the beta coeficient for stock J that is consistent with equilibrium r 12.5 rRF 4.5 rM10.5?
^r = r r = rRF + (rM - rRF) * b b = (r - rRF)/(rM - rRF) b = 1.33
What is rrt do?
Based on Wikipedia, RRT means Registered Respiratory Therapist.
What is RRT?
Based on Wikipedia, RRT means Registered Respiratory Therapist.
How do you calculate relative responce factor in hplc related substance analysis?
(Target ion area response / Int Std area response) * (Int Std Injection Vol / Target Injection Vol)
Would an RRT work in a Cath Lab?
Yes, i am an RRT and i work in a cardiac cath lab.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What services does RRT Global offer?
RRT Global develops technology for oil refineries. The company is headquartered in St. Petersburg, Russia and is best known for developing the PRIS technology for gasoline conversion.
How do you calculate annual risk free rate?
The risk free rate of return is a rate an investor will expect with zero risk over a specified period of time. In order to calculate risk free rate you need to use CAPM model formula ra = rrf + Ba (rm-rrf), where rrf is risk free rate, Ba is beta of security and Rm is market return.
