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RRF= (area or height peak X amount IS)/(area or height X amount pk)

IS is the internal standard

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13y ago

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Related Questions

How you calculate rrf value in hplc methods?

Yes.


What is RRT and RRF in hplc?

In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor


How do you calculate relative responce factor in hplc related substance analysis?

(Target ion area response / Int Std area response) * (Int Std Injection Vol / Target Injection Vol)


How do you calculate annual risk free rate?

The risk free rate of return is a rate an investor will expect with zero risk over a specified period of time. In order to calculate risk free rate you need to use CAPM model formula ra = rrf + Ba (rm-rrf), where rrf is risk free rate, Ba is beta of security and Rm is market return.


What is rs-hplc?

"RS-HPLC method" means "Related Substance HPLC Method".


Beta coefficient Given the following information determine the beta coeficient for stock J that is consistent with equilibrium r 12.5 rRF 4.5 rM10.5?

^r = r r = rRF + (rM - rRF) * b b = (r - rRF)/(rM - rRF) b = 1.33


How do you calculate concentration from peak area in HPLC analysis?

To calculate concentration from peak area in HPLC analysis, you can use the formula: Concentration Peak Area / (Slope x Injection Volume). The peak area is obtained from the chromatogram, the slope is the calibration curve slope, and the injection volume is the volume of the sample injected into the HPLC system.


How do you distinguised np-hplc and rp-hplc?

NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).


Retention time calculation for hplc?

why RT was shifting & how to RT calculation in HPLC


What type of species can be separated by HPLC but not by gas liquid chromatography?

mixture of enantiomers can be separated by HPLC


Where can one purchase HPLC detectors?

You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.


What is dead volume HPLC?

The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.