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RRF= (area or height peak X amount IS)/(area or height X amount pk)

IS is the internal standard

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How do you calculate relative responce factor in hplc related substance analysis?

(Target ion area response / Int Std area response) * (Int Std Injection Vol / Target Injection Vol)


How do you use RF and rrf in Rs calculation?

In the context of calculating resistance (Rs) in a circuit, RF (feedback resistor) and RRF (reference resistor) can be used in feedback amplifier configurations. RF is typically connected in the feedback path between the output and the inverting input of an operational amplifier, while RRF is used to set the gain of the amplifier. The relationship between these resistors can determine the overall gain of the circuit, which in turn affects the voltage drop and thus the calculation of Rs. By applying Ohm's law and the principles of feedback, you can derive Rs based on the values of RF and RRF in the circuit.


What type of species can be separated by HPLC but not by gas liquid chromatography?

mixture of enantiomers can be separated by HPLC


Can melamine detect by HPLC?

Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.


Why volatile compounds are analysed in gc but not in hplc?

GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC

Related Questions

How you calculate rrf value in hplc methods?

Yes.


What is RRT and RRF in hplc?

In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor


How do you calculate relative responce factor in hplc related substance analysis?

(Target ion area response / Int Std area response) * (Int Std Injection Vol / Target Injection Vol)


How do you calculate annual risk free rate?

The risk free rate of return is a rate an investor will expect with zero risk over a specified period of time. In order to calculate risk free rate you need to use CAPM model formula ra = rrf + Ba (rm-rrf), where rrf is risk free rate, Ba is beta of security and Rm is market return.


What is rs-hplc?

"RS-HPLC method" means "Related Substance HPLC Method".


Beta coefficient Given the following information determine the beta coeficient for stock J that is consistent with equilibrium r 12.5 rRF 4.5 rM10.5?

^r = r r = rRF + (rM - rRF) * b b = (r - rRF)/(rM - rRF) b = 1.33


How do you calculate concentration from peak area in HPLC analysis?

To calculate concentration from peak area in HPLC analysis, you can use the formula: Concentration Peak Area / (Slope x Injection Volume). The peak area is obtained from the chromatogram, the slope is the calibration curve slope, and the injection volume is the volume of the sample injected into the HPLC system.


How do you calculate RRF?

The Required Rate of Return (RRF) can be calculated using the Capital Asset Pricing Model (CAPM), which is expressed as: RRF = Risk-Free Rate + Beta × (Market Return - Risk-Free Rate). Here, the risk-free rate is typically the yield of government bonds, beta represents the asset's volatility relative to the market, and the market return is the expected return of the overall market. Alternatively, RRF can also be determined using other methods like the Dividend Discount Model or the Gordon Growth Model, depending on the context and available data.


How do you distinguised np-hplc and rp-hplc?

NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).


Retention time calculation for hplc?

why RT was shifting & how to RT calculation in HPLC


How do you use RF and rrf in Rs calculation?

In the context of calculating resistance (Rs) in a circuit, RF (feedback resistor) and RRF (reference resistor) can be used in feedback amplifier configurations. RF is typically connected in the feedback path between the output and the inverting input of an operational amplifier, while RRF is used to set the gain of the amplifier. The relationship between these resistors can determine the overall gain of the circuit, which in turn affects the voltage drop and thus the calculation of Rs. By applying Ohm's law and the principles of feedback, you can derive Rs based on the values of RF and RRF in the circuit.


What type of species can be separated by HPLC but not by gas liquid chromatography?

mixture of enantiomers can be separated by HPLC