RRF= (area or height peak X amount IS)/(area or height X amount pk)
IS is the internal standard
(Target ion area response / Int Std area response) * (Int Std Injection Vol / Target Injection Vol)
mixture of enantiomers can be separated by HPLC
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
The peak-to-valley ratio in high-performance liquid chromatography (HPLC) is a measure of the separation between the highest peak and the adjacent valleys in a chromatogram. It is calculated by dividing the peak height by the lowest valley height around the peak. A higher peak-to-valley ratio indicates better resolution and a more efficient separation of analytes.
Yes.
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
(Target ion area response / Int Std area response) * (Int Std Injection Vol / Target Injection Vol)
The risk free rate of return is a rate an investor will expect with zero risk over a specified period of time. In order to calculate risk free rate you need to use CAPM model formula ra = rrf + Ba (rm-rrf), where rrf is risk free rate, Ba is beta of security and Rm is market return.
"RS-HPLC method" means "Related Substance HPLC Method".
^r = r r = rRF + (rM - rRF) * b b = (r - rRF)/(rM - rRF) b = 1.33
To calculate concentration from peak area in HPLC analysis, you can use the formula: Concentration Peak Area / (Slope x Injection Volume). The peak area is obtained from the chromatogram, the slope is the calibration curve slope, and the injection volume is the volume of the sample injected into the HPLC system.
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
why RT was shifting & how to RT calculation in HPLC
mixture of enantiomers can be separated by HPLC
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.