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* large columns, specifically for packing. * analytical columns, for quantitative analysis, usually accompanied by a UV-vis detector. * Narrow bore columns, for more sensitive analysis * capillary columns, very this silica columns used almost exclusively with GC mass spectroscopy. * packed bed columns. with silica beads. and may have groups attached, e.g. C18.

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What is difference between uplc and hplc?

A UPLC (ultra-high performance liquid chromatography) is a variant of HPLC using columns with particle size <2 um (typically, 1.8 um), which provides significantly better separation than the traditional (5 um) columns and enables much faster analysis. Strictly speaking, "UPLC" is Waters Corporation trademark, but is often used as a name for the technique in general.


How do you distinguised np-hplc and rp-hplc?

NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).


What has the author Harold M McNair written?

Harold M McNair has written: 'Direct coupling of microbore HPLC columns to MS systems'


How does one apply the HPLC princple?

The HPLC principle is used to separate liquid mixtures into individual parts. It's frequently used in several field such as medicine and law. HPLC is specifically used in sports to test for performance enhancing drugs in urine.


Where can one purchase HPLC detectors?

You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.


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Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.


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