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* large columns, specifically for packing. * analytical columns, for quantitative analysis, usually accompanied by a UV-vis detector. * Narrow bore columns, for more sensitive analysis * capillary columns, very this silica columns used almost exclusively with GC mass spectroscopy. * packed bed columns. with silica beads. and may have groups attached, e.g. C18.
What else can I help you with?
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
What is RRT and RRF in hplc?
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
What is an HPLC column?
HPLC Column is one type of tube containing a stationary phase react with mobile phase to detect peak
What is the definition of method development by hplc?
Method development is a process amenable to continuous improvement
What are the different types of absorption column?
Major types of Absorption columns are liquid-liquid absorption columns, Gas-liquid absorption columns.
What is difference between uplc and hplc?
A UPLC (ultra-high performance liquid chromatography) is a variant of HPLC using columns with particle size <2 um (typically, 1.8 um), which provides significantly better separation than the traditional (5 um) columns and enables much faster analysis. Strictly speaking, "UPLC" is Waters Corporation trademark, but is often used as a name for the technique in general.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
What has the author Harold M McNair written?
Harold M McNair has written: 'Direct coupling of microbore HPLC columns to MS systems'
How does one apply the HPLC princple?
The HPLC principle is used to separate liquid mixtures into individual parts. It's frequently used in several field such as medicine and law. HPLC is specifically used in sports to test for performance enhancing drugs in urine.
Where can one purchase HPLC detectors?
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
What is dead volume HPLC?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
Which are the top commercial uses for HPLC?
HPLC is used to analyze, identify, purify and quantify organic compounds.
What are the key differences between normal phase HPLC and reverse phase HPLC in terms of their separation mechanisms and applications?
Normal phase HPLC separates compounds based on their polarity, with the stationary phase being polar and the mobile phase being nonpolar. Reverse phase HPLC separates compounds based on their hydrophobicity, with the stationary phase being nonpolar and the mobile phase being polar. Normal phase HPLC is typically used for separating polar compounds, while reverse phase HPLC is used for separating nonpolar compounds.
When will discard hplc columns?
HPLC columns should be discarded when their performance declines, indicated by issues such as increased back pressure, poor resolution, or inconsistent retention times. Typically, a column should also be replaced after a specific number of injections, which can vary based on the sample matrix and the conditions used. Regular monitoring of column performance and following manufacturer guidelines can help determine the optimal time for replacement. Additionally, if the column shows signs of physical damage or contamination that cannot be cleaned, it should be discarded.
Which equipment is used to separate mixtures?
HPLC, HPTLC, GPC
