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HPLC columns should be discarded when their performance declines, indicated by issues such as increased back pressure, poor resolution, or inconsistent retention times. Typically, a column should also be replaced after a specific number of injections, which can vary based on the sample matrix and the conditions used. Regular monitoring of column performance and following manufacturer guidelines can help determine the optimal time for replacement. Additionally, if the column shows signs of physical damage or contamination that cannot be cleaned, it should be discarded.
What else can I help you with?
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
What is C18 phase in hplc column?
Both C8 and C18 columns are considered as examples of reversed phase liquid chromatography (RP). The stationary phase here is seen as a thin film of non-polar liquid phase that has been designed to be chemically anchored to an inert material (Silica gel particles). The non-polar layer is chemically linked to the silica particles surface by reaction with the polar silanol groups on the stationary phase surface and so rendering them (less polar or non-polar), The difference between the two columns will be in the length of the carbon-chain attached to the silica surface. Acoordingly C8 hplc columns have packing material composed of silica particles attached to C8 carbon units while C18 will, of course, have packing materials coated with C18 hydrophobic units. Categorically both are reversed phase but C18 columns will definitely be more "hydrophobic rather than the C8 columns.
Which are the top commercial uses for HPLC?
HPLC is used to analyze, identify, purify and quantify organic compounds.
What are the columns used in hplc?
* large columns, specifically for packing. * analytical columns, for quantitative analysis, usually accompanied by a UV-vis detector. * Narrow bore columns, for more sensitive analysis * capillary columns, very this silica columns used almost exclusively with GC mass spectroscopy. * packed bed columns. with silica beads. and may have groups attached, e.g. C18.
What has the author Harold M McNair written?
Harold M McNair has written: 'Direct coupling of microbore HPLC columns to MS systems'
What is difference between uplc and hplc?
A UPLC (ultra-high performance liquid chromatography) is a variant of HPLC using columns with particle size <2 um (typically, 1.8 um), which provides significantly better separation than the traditional (5 um) columns and enables much faster analysis. Strictly speaking, "UPLC" is Waters Corporation trademark, but is often used as a name for the technique in general.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What is the difference ods and ods2 HPLC Column?
BDS - Base deactivated Silanol ODS - Octadecyl silane by using the BDS column, residual silanols deacivated and silanol activity reduced, it is end-capped column and it is good for basic compounds. ODS is normal column and high acidic silica, it is not suitable for basic compounds.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
Where can one purchase HPLC detectors?
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.
What is C8 and C18?
C8 and C18 refer to carbon chain lengths in fatty acids. C8 means the fatty acid has 8 carbon atoms in its chain, while C18 means the fatty acid has 18 carbon atoms in its chain. The number of carbon atoms in a fatty acid chain can affect its properties and functions in the body.
What disadvantage does the high performance liquid chromatography have?
The columns are expensive, electrical glitchs cause instrument failure, bubbles in the lines result in no pressure and bad check valves....... The list can go on, but, HPLC is very good instrumentation if you know what you are doing.
What is RRT and RRF in hplc?
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
