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The chemical formula of dithyothreitol is C4H10O2S2. As applications it is a reducing agent.
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∙ 11y ago
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What is another solvent that can be used instead of beta-mercaptoethanol?
dithiothreitol
What is the function of dithiothreitol in DNA extraction?
DTT is the reducing agent for thiolated DNA
What is denaturing sds page?
Sodium dodecyl sulphate (SDS) is an anionic detergent which denatures proteins by "wrapping around" the polypeptide backbone - and SDS binds to proteins fairly specifically in a mass ratio of 1.4:1. In so doing, SDS confers a negative charge to the polypeptide in proportion to its length - ie: the denatured polypeptides become "rods" of negative charge cloud with equal charge or charge densities per unit length. It is usually necessary to reduce disulphide bridges in proteins before they adopt the random-coil configuration necessary for separation by size: this is done with 2- mercaptoethanol or dithiothreitol. In denaturing SDS-PAGE separations therefore, migration is determined not by intrinsic electrical charge of the polypeptide, but by molecular weightAnd for the actual experiment beyond the denaturing: PAGE stands for polyacylamide gel electrophoresis. This is a procedure that separates proteins by size by running them through a gel "matrix" so that the smaller ones travel faster that the larger ones. This is achieved by creating an electric field with the sds-protein complex traveling toward the positively charged end of the gel. Once the smallest proteins have traveled most of the way across the gel the current is turned of and the gel is removed and stained with dye that binds protein so that you can see where it is in the gel.
