There is a flow cytometry page on Wikipedia which contains information on the history, principle, data analysis, labelling and applications of flow cytometry. Invitrogen is another website where you can find information.
Virginia M. Litwin has written: 'Flow cytometry in drug discovery and development' -- subject(s): Flow cytometry, Design, Methods, Drugs, Flow Cytometry, Pharmaceutical Chemistry, Drug Design
Flow Cytometry
flow cytometry
Flow Cytometry: CD41+ /Tiazole orange = 6-10% from total platelet population. ELena
Flow cytometry is a method for quarantining components or structural features of cells primarily by optical means. Although it makes measurements on one cell at a time, it can process thousands of cells in a few seconds. Since different cell types can be distinguished by quantitating structural features, flow cytometry can be used to count cells of different types in a mixture. Flow cytometers have been commercially available since the early 1970's and their use has been increasing since then. The most numerous flow cytometers are those used for complete blood cell counts in clinical laboratories - - these do not employ fluorescence. Lymphomas and leukemia's are intensively studied for surface markers of diagnostic and prognostic value. Although less expensive alternative technologies are under development, until the present time, flow cytometry has been the method of choice for monitoring CD4 lymphocyte levels in the blood of AIDS patients.
Computers can help scientists learn about bacteria any time there is a need for number crunching which occurs extremely frequently when scientists study bacteria. Flow cytometry, for example, can count the number of bacteria in a sample, using lazers and detectors. The electronic information from the detectors is fed into a computer where it is analyzed and turned into useful information about how many bacteria are present. Without the computer, flow cytometry and many other complex technologies useful for studying bacteria would be impossible.
One test uses flow cytometry light scatter analysis; this method can evaluate a sample of infected serum in as little as 90 minutes. A second technique is an IgM-enzyme-linked immunosorbent assay (ELISA), which detects the presence of IgM antibodies
Cytometry is the medical term meaning process of measuring or counting cells.
White blood cells have a distinct forward and side scatter pattern. You can see this by doing flow cytometry on a blood sample. The white blood cells can then be separated by using a FACSorter.
North America Minimal Residual Disease (MRD) Testing Market 2021-2031 by Offering (Services, Test Kits & Consumables), Application (NHL, MM, ALL, CLL, AML, CML, HL, Solid Tumor), Technology (Flow Cytometry, PCR, NGS, Others), End User (Hospitals and Clinics, Research Institutions, Diagnostic Laboratories, Others), and Country: Trend Forecast and Growth Opportunity.
The unit of measurement for bacteria is micrometer (µm). Several methods have been used now and in the past to estimate average bacterial size: 1. electron microscopy, 2. electronic devices (Coulter counter and flow cytometry), and 3. epifluorescence microscopy. Transmission electron microscopy is tedious and expensive because samples require long and elaborate processing before analysis. Scanning electron microscopy is relatively faster and cheaper, but processing of the samples may produce cell shrinkage and underestimation of bacterial size. Coulter counters have a very low resolution in the bacterial size range and do not discriminate between bacteria and dead or inert particles. Flow cytometry has similar limitations. The most popular method for bacterial size measurement relies on epifluorescence images. Direct computerized image analysis of the epifluorescence samples is the most precise and fastest method currently.