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The capacity factor, k' is the same in all chromatography, except in Micellar Electrokinetic capillary chromatography (MEKC).
k' = (tR - tM)/ tM
Where:
tR = retention time (time between injection and elution)
tM = tR of the unretained species (ie. how long it took for the solution you injected to travel all the way through the length of the column). tM is ALWAYS smaller than tR.
The Capacity factor has been changed officially to the retention factor by IUPAC recently
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Rephrase your question so that it makes sense.
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What is the use of calculating asymmetry factor in hplc?
it is used to check the efficiency of the chromatography column used
How many grams are in 18 mls?
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