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RRF= (area or height peak X amount IS)/(area or height X amount pk)
IS is the internal standard
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How do you calculate relative responce factor in hplc related substance analysis?
(Target ion area response / Int Std area response) * (Int Std Injection Vol / Target Injection Vol)
Peak-to-valley ratio in HPLC?
to calculate signal to noise use this formulae peak hight *2/noise level
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
How you calculate rrf value in hplc methods?
Yes.
What is RRT and RRF in hplc?
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
How do you calculate relative responce factor in hplc related substance analysis?
(Target ion area response / Int Std area response) * (Int Std Injection Vol / Target Injection Vol)
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
How do you calculate annual risk free rate?
The risk free rate of return is a rate an investor will expect with zero risk over a specified period of time. In order to calculate risk free rate you need to use CAPM model formula ra = rrf + Ba (rm-rrf), where rrf is risk free rate, Ba is beta of security and Rm is market return.
Beta coefficient Given the following information determine the beta coeficient for stock J that is consistent with equilibrium r 12.5 rRF 4.5 rM10.5?
^r = r r = rRF + (rM - rRF) * b b = (r - rRF)/(rM - rRF) b = 1.33
Peak-to-valley ratio in HPLC?
to calculate signal to noise use this formulae peak hight *2/noise level
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
How do you calculate lod and loq by hplc give example?
refer ICH chapter Q2(R1) for beter underrstsnding. Dnyaneshwar Patil
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
