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Methods involved in moist heat sterilization below hundred degree celsius?
WELL! moist heat is used by heating with steam and the steam can not be formed below 100oC so it is not possible to kill microorganism by moist heat below hundred degree
What is the relationship of time to temperatures in heat sterilization?
Dry heat = 1-2hrs @ 160-170 degrees Celsius Moist heat = 15min. @ 121 degrees Celsius
What is the definition of moist heat sterilization?
Dry-heat sterilization is generally a less complicated process than steam sterilization; it is, however, relatively slow and requires higher temperatures and/or longer exposure times. This is because of the fact that microbial lethality is lower with dry heat than that for steam at the same temperature.
Is cold sterilization the same as chemical sterilization?
Yes. Chemical sterilization and cold sterilization are the same thing.
Why are culture media not sterilized in hot air sterilization?
Culture media are not sterilized using hot air sterilization because this method primarily targets dry materials and is less effective for moisture-rich substances. The high temperatures required can degrade heat-sensitive components in the media, potentially compromising their effectiveness. Instead, autoclaving or steam sterilization is preferred for culture media as it utilizes moist heat to achieve effective sterilization without damaging the nutrients essential for microbial growth.
Why Moist heat sterilization is more effective than Dry heat sterilization?
Mechanism of killing by dry heatDry heat kills the organisms by destructive oxidation of essential cell constituentsKilling of the most resistant spores by dry heat requires a temperature of about 160 °C for 60 minutesDry heat is employed for glassware; syringes, metal instruments and paper wrapped goods, which are not spoiled by high temperatures.It is also used for anhydrous fats, oils and powders that are impermeable to moisture.Mechanism of killing by moist heatMoist heat kills the organisms by coagulating and denaturing their enzymes and structural protein.Sterilization by moist heat of the most resistant spores generally requires 121 °C for 15-30 minutes.Moist heat is used for the sterilization of culture media, and all other materials through which steam can penetrateMoist heat is more effective than dry heatSterilization can be done at lower temperatures in a given time at a shorter duration at the same temperature.upon heating wet proteins release free -sh groups and form smaller peptide chains which in turn form new complexes different from the orignal protein molecules
What is gas sterilization?
This is a procedure of sterilization using a gas.
What is the sterilization of women?
This is the mandatory sterilization of abnormal or undesirable women.
What is sterilization process?
The sterilization process is to have something completely clean
What has the author William M Moss written?
William M. Moss has written: 'Contraceptive sterilization' -- subject(s): Contraception, Sexual Sterilization, Sterilization (Birth control), Tubal sterilization, Vasectomy
What is the mean of gas sterilization on general?
This is a procedure of sterilization using a gas.
Spray-wipe and the ultrasonic cleaner will?
accomplish sterilization.
