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Q: What is optical density on a ELISA standard curve?
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What is the difference between a probability density curve and cummulative distribution function?

what is density curve


Is normal distribution symmetrical?

Yes. By definition. A normal distribution has a bell-shaped density curve described by its mean and standard deviation. The density curve is symmetrical(i.e., an exact reflection of form on opposite sides of a dividing line), and centered about (divided by) its mean, with its spread (width) determined by its standard deviation. Additionally, the mean, median, and mode of the distribution are equal and located at the peak (i.e., height of the curve).


Does a curved mirror necessarily have optical power?

Yes but a complex curve will not have a simple or single optical power.


Is the standard normal curve non symmetrical?

The standard normal curve is symmetrical.


What is the area under the standard normal curve?

the standard normal curve 2


He area under the standard normal curve is?

The area under the standard normal curve is 1.


What does the total area under a density curve equal?

The integral of the density with respect to the variable against which the density is plotted, between the values at the ends of the curve. Since there is no information given as to what the density is plotted against, a more informative answer is impossible.


How do you prepare the standard curve?

To prepare the standard curve you will need linear graph paper, semi-log graph paper and absorbance. You can define your standard curve by finding the absorption or percent plot on the Y axis.


Why is the ELISA test so sensitive?

It has many reason that may be possible. 1. Stability of Elisa kit. 2. Type of Elisa kit ... Example. Rat IL-1 must use with cell rat .... but can use with the other depend cross reactivity of Elisa kit. 3. Right method will show good standard curve. (r2 almost 1 ) if not it's wrong. 4. The concentration used of treated in cell is not appropiate .


What are the properties of a standard normal curve?

It is a normal curve with mean = 0 and variance = 1.


How do you make a standard curve graph?

Standard curves are used to determine the concentration of substances. First you perform an assay with various known concentrations of a substance you are trying to measure. The response might be optical density, luminescence, fluorescence, radioactivity or something else. Graph these data to make a standard curve - concentration on the X axis, and assay measurement on the Y axis. Also perform the same assay with your unknown samples. You want to know the concentration of the substance in each of these unknown samples. To analyze the data, fit a line or curve through the standards. For each unknown, read across the graph from the spot on the Y-axis that corresponds to the assay measurement of the unknown until you intersect the standard curve. Read down the graph until you intersect the X-axis. The concentration of substance in the unknown sample is the value on the X-axis. In the example below, the unknown sample had 1208 counts per minute, so the concentration of the hormone is 0.236 micromolar. Prism makes it very easy to fit your standard curve, and to read (interpolate) the concentration of unknown samples.


What is the mean and standard deviation for the standard normal curve?

Mean = 0 Standard Deviation = 1