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It has many reason that may be possible. 1. Stability of Elisa kit. 2. Type of Elisa kit ... Example. Rat IL-1 must use with cell rat .... but can use with the other depend cross reactivity of Elisa kit. 3. Right method will show good standard curve. (r2 almost 1 ) if not it's wrong. 4. The concentration used of treated in cell is not appropiate .
What else can I help you with?
What blot test is used to confirm a seropositive ELISA test for HIV?
The western blot test
What type of test is a ELISA?
an Enzyme Linked immunosorbent assay. during an infection an individual mounts an antibody response which eventually results in production of plasma IgG molecules that bind to various parts of the infectious agent. If these antibodies are present in the sample they will bind to the adsorbed antiagen and remain there after washing and will be detected by the ELISA technique.
What are the disadvantages of the ELISA test?
two advantages are you don't need to use radioactive substances and it five accurate results highly sensitive and specific not needing radioisotopes (radioactive substances) or a costly radiation counter (a radiation-counting apparatus)
What is indirect elisa?
In the Indirect ELISA ,An antigen is added to the microtiter plate well and the antigen attaches to the walls of the microtiter plate.After rinsing to remove excess antigen, the serum suspected of containing the antibodies is added.Enzyme-linked antibody capable of reacting with the constant region of other antibodies is the added, followed by addition of the colorless substrate. Development of color indicates the presence of the antibody being identified.
What does the name Elisa mean?
Elisa means "consecrated to God"
Why is the secondary antibody used in an ELISA test conjugated with an enzyme?
The secondary antibody in an ELISA test is conjugated with an enzyme to amplify the signal produced when the antibody binds to the target antigen. This enzyme-substrate reaction generates a detectable signal that indicates the presence of the antigen, which allows for more sensitive and accurate detection in the ELISA assay.
What is the first test used for detecting HIV what happens if the results of this test are reactive?
The first test performed is ELISA (or EIA) is a test that screens for the presence of HIV antibodies in the blood. This test is not a test for HIV/AIDS its a very sensitive and will be reactive even if there are only 1 or 2 antibodies in that person blood sample. If the results are reactive that test will be followed by a second test called confirmatory test which is highly accurate test in which it's is used to confirm the results of the ELISA.
What are the differences between sandwich ELISA and direct ELISA?
Sandwich ELISA uses two antibodies to detect an antigen, while direct ELISA uses only one antibody. Sandwich ELISA is more sensitive and specific, but direct ELISA is simpler and faster.
Why is the Elisa test so sensitive and specific?
The ELISA (Enzyme-Linked Immunosorbent Assay) test is sensitive and specific due to its ability to utilize antibodies that bind to specific antigens, allowing for the detection of even low concentrations of target molecules. The test employs a solid surface to immobilize the antigen or antibody, enhancing the stability and accessibility of the target. Additionally, the use of enzyme-linked detection amplifies the signal, increasing sensitivity. Overall, the combination of high affinity antibodies and signal amplification contributes to its reliability and accuracy in diagnosing various conditions.
The first test given to determine that presence of HIV?
The ELISA test is usually the first test given; quick results and inexpensive. See the related link.
When testing for HIV what tests produce more accurate results than the ELISA test?
Western Blot Test: is a blood test that produces more accurate results than the ELISA test.
What HIV test produces more accurate results than ELISA test?
There is only one test that can give the accurate results for HIV. The only test that can do this would be the Elisa test.
What is the agid and elisa test?
The AGID test is the agar gel immunodiffusion test and ELISA is the enzyme-linked immunosorbent assay. Both test for the presence of certain proteins (called antigens) by binding them with antibodies.
What is meant by elisa and its uses?
An Elisa Test is used to test for a substance using antibodies for colour change. The test has been used in medicine and plants. Performing the test involves using an antibody and enzymes.
The confirmatory test for AIDS is?
elisa
Is it possible for a person to be Elisa positive but Western blot negative for a certain condition?
Yes, it is possible for a person to test positive for the Elisa test but negative for the Western blot test for certain conditions.
Use to comfirm a seropositive ELISA test for HIV?
western blot test
