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What occurs first in the production of a recombinant plasmid?
Isolation of a plasmid from a bacterium
Why you use LiCl in plasmid isolation by telt method?
helps in DNA ppt
Role of Glacial acetic acid in plasmid isolation?
to neutralise the alkaline conditions.
Function of phenol chloroform in plasmid isolation?
removes the remaining protein which is left after denaturation
What is the function of phenol chloroform isoamyl alcohol in plasmid isolation?
it helps in the removal of proteins from nucleic acid
What is the Role of RNAase in plasmid preparation?
In plasmid isolation RNA behaves as an unwanted material so to separate it out RNAase is required which breaks down the RNA. This is done to get pure quality of the product.
The function of Wash solution in plasmid DNA extraction?
Plasmid isolation has a step called washing step that carried out in the column in which the plasmid DNA are already bind. There are two wash solution, first one endo wash buffer that wash the traces of bacterial membrane remnants such as LPS. Wash buffer two has ethanol wash off any protein contaminants present on the column. These wash steps ensure the purify of isolated plasmid DNA.
What is the use of alkaline lysis solution 1 in plasmid isolation?
it helps to homogenize the cell and give single cell suspension
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
Role of peg in plasmid DNA isolation?
it reduces the availability of solvents. that is dissociation of H+ ond OH- ions takes place. which makes the macromoleules to condence or crowd. and let to precipitate
What is the function of the Ti plasmid?
what is the function of the plasmid
Role of NaOH in plasmid DNA isolation?
Plasmid isolation involves growing the plasmid under conditions that are suitable for genes to come into play. For example the gene for ampicillin resistance; the bacteria with plasmids are placed with ampicillin so their genes can be seen for those who survived. Sodium hydroxide acts a detergent in the extraction process. A detergent's main role is to break down cell walls and cell membranes. How so? They act as poking holes into membranes. However, for the isolation of plasmid, the NaOH acts
