0
Want this question answered?
Be notified when an answer is posted
Add your answer:
Earn +20 pts
How do you differentiate urobilinogen and porphobilinogen?
Watson-Schwartz test negative : urobilinogen Watson-Schwartz test positive : porphobilinogen
What are the common interfering substances for porphobilinogen assay?
The interfering substances for porphobilinogen assay includes urea and urobilinogen. Since they can affect the results of the assay, interfering substances are removed by selectively adsorbing PBG onto an ion-exchange resin (PubMed, 1989).
Does Cola has a PH of 2.5?
yeah, it is reported in tests conducted at the Maakeloude University at southern Germany. However cocacola dismissed these claims as rubbish. Some speculators claim that the actual pH could well be below 2.5.The university findings show that under the action of porphobilinogen synthase the OH- radical is generated by aminolevulinate. Structure of porphobilinogen deaminase reveals a flexible multidomain polymerase with a single catalytic site. This multidomain flexibility gives humans the feeling of quenching of thirst. But hydroxymethylbilane synthase is an enzyme involved in the third step of the metabolism of porphobilinogen which decreases the pH to values below 2.75.
PBG test?
DefinitionPorphobilinogen (PBG) is one of several types of porphyrins found in your body. Normally, your body breaks down porphyrins into heme, an important part of hemoglobin. Porphyrins usually leave your body through urine or stools. If this process is interrupted, porphyrins such as PBG can build up in your body.This article describes the test to measures the amount of PBG in a urine sample.Alternative NamesPorphobilinogen testHow the test is performedA 24-hour urine sample is needed. The health care provider will instruct you, if necessary, to stop taking drugs that may interfere with the test.On day 1, urinate into the toilet when you get up in the morning.Afterwards, collect all urine in a special container for the next 24 hours.On day 2, urinate into the container when you get up in the morning.Cap the container. Keep it in the refrigerator or a cool place during the collection period.Label the container with your name, the date, the time of completion, and return it as instructed.For an infant, thoroughly wash the area around the urethra. Open a urine collection bag (a plastic bag with an adhesive paper on one end), and place it on the infant. For males, the entire penis can be placed in the bag and the adhesive attached to the skin. For females, the bag is placed over the labia. Diaper as usual over the secured bag.This procedure may take a couple of attempts -- lively infants can displace the bag, causing the specimen to be absorbed by the diaper. The infant should be checked frequently and the bag changed after the infant has urinated into the bag. The urine is drained into the container for transport to the laboratory.Deliver it to the laboratory or your health care provider as soon as possible upon completion.How to prepare for the testYour doctor may tell you to stop taking certain drugs that may interfere with test results.Drugs that can affect test results include:Aminosalicylic acidBarbituratesBirth control pillsChloral hydrateChlorpropamideEthyl alcoholGriseofulvinMorphinePhenazopyridineProcaineSulfonamidesNever stop taking any medicine without first talking to your doctor.How the test will feelThis test involves only normal urination, and there is no discomfort.Why the test is performedThis test may be performed when porphyria or another disorder associated with an abnormal porphobilinogen (PBG) level is suspected.Normal ValuesFor a random urine sample, a negative test result is considered normal.If the test is done on a 24-hour urine sample, the normal value is less than 4 milligrams per 24 hours.Note: Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.What abnormal results meanIncreased levels of PBG in the urine may be due to:HepatitisLead poisoningLiver cancerPorphyria(several types)What the risks areThere are no risks.ReferencesAnderson KE. The porphyrias. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier;2007:chap 229.
