Quantitative PCR Technology is used in biochemistry, in particular molecular Biology. The PCR stands for polymerase chain reaction and is used to "amplify" pieces of DNA to make millions of copies of a particular DNA strand.
: Differentiate between quantitative and real time PCR.
Certainly rt-PCR is qualitative and can also theoretically be quantitative. Anneal the RNA to get a 1:1 RNA to DNA copy, then proceed with quantitative PCR.
PCR technology can be used to this field.
my HBV VIRAL LOAD BY REAL TIME PCR IS 165 MEANS
In qualitative PCR specific DNA fragment is detected while in quantitative PCR our target DNA sequence not only is detected but its amount is determined (after reaction we can calculate the amount of DNA we had in our sample)
PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.
Recombinant DNA technology PCR
r DNA technology is technology of creating new combination of DNA. While pcr is one of techniques used in r DNA technology for amplification of perticuler DNA fragment
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
PCR
It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
The basic principles of quantitative real time PCR include having the ability to target a particular DNA or RNA sample in a sample relative to either a standard or another sample that has been subjected to treatment.