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What is the purpose of RNA extraction?
RNAse destroys the RNA and hence RNAse contamination is a problem in RNA extraction as it breaks down RNA. RNAse enzyme is removed by using RNAse inhibitor or precautions like wearing of gloves, autoclaving tips , using RNAse free water/DEPC treated water is done while performing RTPCR
What is the role of DEPC in RNA isolation?
It inactivate the RNase and prevent RNA to denature.
What is superasin?
An RNAse inhibitor, made by Ambion http://www.ambion.com/techlib/resources/superasein/
What are free water molecules?
a free molecule iun water
What epidermal cell defends skin against microbes?
i don't know about chemicals but epidermis cells produce RNase enzymes to destroy microbial genomes
What is the purpose of RNA extraction?
RNAse destroys the RNA and hence RNAse contamination is a problem in RNA extraction as it breaks down RNA. RNAse enzyme is removed by using RNAse inhibitor or precautions like wearing of gloves, autoclaving tips , using RNAse free water/DEPC treated water is done while performing RTPCR
Role of RNase in DNA purification?
RNAase remove RNA from the solution as we need DNA only. RNAse cuts down the RNA strand.
What is the role of DEPC in RNA isolation?
It inactivate the RNase and prevent RNA to denature.
What does RNase solution do in the Isolation of genomic DNA from Negative bacteria?
Digests RNA molecules
What is superasin?
An RNAse inhibitor, made by Ambion http://www.ambion.com/techlib/resources/superasein/
What is the role of TRIreagent in RNA isolation?
Break open the cells, stabilize RNA, inhibit RNAse.
What is the recipe for Lysis buffer?
Bacterial lysis buffer: 1mL 1M Tris-HCl pH 8.0 200uL 0.5M EDTA 15g sucrose (add to water, not the other way around) 200mg lysozyme 20mg pancreatic RNase 10mg BSA Bring up to 100mL with water filter sterilize (do not autoclave)
How much water on earth is free water?
What free water? are you meaning fresh or salt water?????????
Why RNA extraction is difficult?
RNA itself difficult to handle because of its unstable nature. Unlike DNA, RNA has free 2'-OH group in their ribose sugar that make them highly reactive. Other than this, RNAse contamination is everywhere (during isolation RNAase from our skin can kill RNA).
What are free water molecules?
a free molecule iun water
What epidermal cell defends skin against microbes?
i don't know about chemicals but epidermis cells produce RNase enzymes to destroy microbial genomes
Why is underground water free from germs?
It isn't free of germs and that is why we purify the water.
