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The addition of calcium chloride to a cell suspension promotes the binding of plasmid DNA to Lipopolysaccharide. Positively charged calcium ions attract both the negatively charged DNA backbone and the negatively charged groups in the Lipopolysaccharide inner core. The plasmid DNA can then pass into the cell upon heat shock, where cells are cooled to a low temperature (+4 degrees Celsius) and then heated to a high temperature (+42 degrees Celsius) for a short time.

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Q: What is roll of calcium chloride in making competent cells in transformation?
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After making standard calcium chloride solution how you can adjust the 1ml equals 1 mg of calcium?

It all depends on the type of Calcium Chloride you used. Was it anhydrous or dihydrate or else?


How can I write the balanced chemical reaction for making CaCl2?

You could add hydrochloric acid to calcium to form calcium chloride and hydrogen gas. 2HCl + Ca → CaCl2 + H2


How do you covert sodium chloride to sodium metal?

Mix with calcium chloride, melt at 1300 F, use electrolysis. A glass making microwave kiln capable of that temperature sells for about $100.


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no, calcium does not contain carbon, making it inorganic


What is the trace element most important for making bones hard?

Calcium


What if you used water softener in your salt water pool?

Filling a plaster pool with water from a salt type softener will severely damage the plaster. Salt water softeners exchange salt for calcium and leave the water in a calcium deficiency. Seeking its dynamic balance the water will leech calcium from the plaster to satisfy its need for calcium making the plaster etched and rough. Adding salt to regular tap water is not the same as filling with soft water as the calcium is still present in the tap water. 180 ppm of calcium is considered the minimum and low calcium can be raised by adding calcium chloride.


Energy transformation making toast in an electric toaster?

Heat energy


What is important for making and maintaining bone?

Calcium


What is used in making plastic?

Polyvinyl Chloride... i guess


What is the word equation for the making of concrete?

calcium hydroxide + carbon dioxide = calcium carbonate + water


Will gypsum fizz from hydrochloric acid?

Gypsum (also known as CaSO3) and Hydrochloric acid (also known as muriatic acid) creates the following reaction: CaSO3 + 2HCl ---> CaCl2 + H2O + SO2 You should see the CaSO3 dissolve in the HCl assuming the HCl is strong enough in molarity or % concentration. Try it at home with some simple sidewalk chalk (the most common form of Gypsum) and HCl. Let it react and then boil away the water and sulfur dioxide to gain calcium chloride, a substance commonly used in home cheese making.


What are the uses of alkaline earth metal?

Magnesium oxide is used in the lining of furnaces. Magnesium hydroxide is used in making of anti-acid tablets. Calcium chloride is used in the extraction of sodium metal,to lower the melting point of NaCl. Calcium sulphate is used as plaster of paris to set fractured bones. Calcium oxide is used as a drying agent,is also used to raise the soil pH.