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What is the definition of competent cells and how it can help to make a transformation more efficient during the cloning of desired gene?
Generally most of the bacteria have the ability to take up DNA which is present freely in the environment. This is called Competence. It is genetically acquired. But some cells do not exhibit natural competence and they are treated with a suitable chemical like CaCl2 to make their cell wall relatively permeable to DNA. We can make any cell competent in vitro and introduce our desired gene which is taken up by the competent cell. To test the efficiency of our treatment, we may introduce a marker gene like gene for antibiotic resistance such as Tetracyclin onto the desired gene and grow the treated cells on Tetracyclin medium so that only the cells that have taken up the DNA survive.
What is the treatment for precancerous cells?
Treatment for precancerous cells may include close monitoring (observation), medication to stop the cells from becoming cancerous, or procedures to remove the abnormal cells (such as surgery, cryotherapy, or laser therapy). The specific treatment will depend on the type of precancerous cells and the individual situation, so it's important to discuss options with a healthcare provider.
Are ciliated metaplasia dangerous?
Ciliated metaplasia itself is not considered dangerous. It is a reversible change in which damaged cells are replaced with ciliated columnar cells, typically in response to irritation or inflammation. However, the underlying cause of the metaplasia may require treatment to prevent further damage.
What discovery did not contribute to cells theory?
All cells require ATP for energy
What is a big disadvantage of using uni-potent cells for medical treatment?
A significant disadvantage of using uni-potent cells for medical treatment is their limited differentiation potential, as they can only develop into one specific cell type. This restricts their applicability in regenerative medicine and tissue repair compared to pluripotent or multipotent cells, which can generate multiple cell types. Consequently, therapies based on uni-potent cells may require more complex procedures or combinations with other cell types to achieve desired therapeutic outcomes.
Can cells be transformed when they are in a competent state?
Yes, cells can be transformed when they are in a competent state. Competent cells have an increased ability to take up foreign DNA, making them more likely to undergo transformation when exposed to external DNA molecules. This process is commonly used in laboratories for genetic engineering and cloning.
What is the definition of competent cells?
cells which readily accept a foreign DNA through a process called transformation
What is meant by competent cells?
Competent cells are bacterial cells that have been treated to make them capable of taking up foreign DNA, such as plasmids. These cells can be used for various genetic engineering techniques, such as transformation.
How can Dr's refuse medical treatment for serious heart issues?
Any competent patient, including a cardiologist, can choose not to get treatment for any condition.
What treatment do serious jaw fractures require?
More serious fractures require complicated, multi-step treatment.
What does Cells require to burn up food?
cells require oxygen to burn sugar.
Where is there a drug rehab that specializes in treatment of Oxycontin?
Treatment for all opioid drugs, from Oxycontin to heroin is the same. Any competent treatment center can do it. Look for one that offers in-house Suboxone detox or the equivalent.
How does cancer treatment affect healthy cells?
Cancer treatment affects healthy cells by killing them , because the keno therapy has radiation and it kills your cells.
What types of cells require energy?
All of your cells require energy. Without energy you wouldn't be alive.
When can people refuse treatment for themselves or others?
When a person is competent and is not intent to do self-harm, he or she can refuse treatment. A person designated to make medical decisions for another person who is unconscious and cannot speak for themselves can direct treatment.
What is meant by term competent when applied to bacteria being prepared for transformation?
Being competent refers to the ability of bacteria to uptake foreign DNA. In the context of transformation, bacteria are made competent through the use of special techniques that temporarily disrupt their cell walls, allowing foreign DNA to enter the cells. This process is essential for the successful transfer of new genetic material into bacterial cells.
What is the definition of competent cells and how it can help to make a transformation more efficient during the cloning of desired gene?
Generally most of the bacteria have the ability to take up DNA which is present freely in the environment. This is called Competence. It is genetically acquired. But some cells do not exhibit natural competence and they are treated with a suitable chemical like CaCl2 to make their cell wall relatively permeable to DNA. We can make any cell competent in vitro and introduce our desired gene which is taken up by the competent cell. To test the efficiency of our treatment, we may introduce a marker gene like gene for antibiotic resistance such as Tetracyclin onto the desired gene and grow the treated cells on Tetracyclin medium so that only the cells that have taken up the DNA survive.
