0
What else can I help you with?
What is meant by competent cells?
Competent cells are bacterial cells that have been treated to make them capable of taking up foreign DNA, such as plasmids. These cells can be used for various genetic engineering techniques, such as transformation.
What are some types of cells that have been transformed?
Some types of cells that have been transformed include cancer cells, stem cells, and bacterial cells for genetic engineering purposes. Transformation can involve altering the genetic material of a cell to express different characteristics or functions.
What is meant by term competent when applied to bacteria being prepared for transformation?
Being competent refers to the ability of bacteria to uptake foreign DNA. In the context of transformation, bacteria are made competent through the use of special techniques that temporarily disrupt their cell walls, allowing foreign DNA to enter the cells. This process is essential for the successful transfer of new genetic material into bacterial cells.
Why do you keep competent cells in ice?
Competent cells are stored in ice to maintain their viability and stability. Storing them at low temperatures helps prevent degradation of their membrane and genetic material, ensuring that they remain suitable for genetic transformation experiments.
Why is it necessary to autoclave transformed cells immediately after experiment?
It is necessary to autoclave transformed cells immediately after the experiment to prevent their unintentional release into the environment, where they could potentially reproduce and propagate. Autoclaving helps to ensure proper containment and disposal of genetically modified organisms, reducing the risk of unintended environmental impact.
Plasma cells develop from transformed t or b cells?
The plasma cells develop from transformed b cells.
What enzyme is produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18?
The enzyme produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18 is luciferase. This enzyme is responsible for the bioluminescent properties of animals like fireflies and glowworms. Cells transformed with plasmid lux will emit light in the presence of the substrate luciferin, whereas cells transformed with pUC18 will not.
Plasma cells develop from transformed b cells or t cells?
ativated B cells forms effector cells which are called plasma cells
What is the definition of competent cells?
cells which readily accept a foreign DNA through a process called transformation
What is meant by competent cells?
Competent cells are bacterial cells that have been treated to make them capable of taking up foreign DNA, such as plasmids. These cells can be used for various genetic engineering techniques, such as transformation.
Can transformed cells pass their newly- acquired traits?
yes
Suppose you transform bacteria with a recombinant DNA plasmid and by mistake grow the transformed cells without an antibiotic . What result would you observe?
If you grow transformed bacteria containing a recombinant DNA plasmid without an antibiotic, you would likely observe that only a small proportion of the cells that successfully took up the plasmid will survive, while the majority of non-transformed cells will also grow. However, the transformed cells may not express the gene of interest or provide any selective advantage, resulting in no significant difference in growth compared to the non-transformed cells. Over time, the population would likely consist mostly of non-transformed cells, as they do not require any selective pressure to thrive.
What treatment do cells require to be competent?
this depends on whether you are trying to make chemically competant cells or electrically competant cells. the process of making a cell "competant" means that they are capable of accepting 'donor' DNA
What are some types of cells that have been transformed?
Some types of cells that have been transformed include cancer cells, stem cells, and bacterial cells for genetic engineering purposes. Transformation can involve altering the genetic material of a cell to express different characteristics or functions.
What is a reservoir for lymphocytes that can be transformed into specific disease fighting cells?
my pp
What is one way that water is transformed?
Water can be transformed by freezing it into ice, which changes its physical state from liquid to solid.
What is meant by term competent when applied to bacteria being prepared for transformation?
Being competent refers to the ability of bacteria to uptake foreign DNA. In the context of transformation, bacteria are made competent through the use of special techniques that temporarily disrupt their cell walls, allowing foreign DNA to enter the cells. This process is essential for the successful transfer of new genetic material into bacterial cells.
