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A population of cells carrying a desired plasmid is called a?
A population of cells carrying a desired plasmid is called a transformed population.
How does a human insulin genes become part of a plasmid?
1. Scientists remove plasmids, small rings of DNA, from bacterial cells. 2. An enzyme cuts open the plasmid DNA. The same enzyme removes the human insulin gene from its chromosome. 3. The human insulin gene attaches the open ends of the plasmid to form a closed ring. 4. Some bacterial cells take up the plasmids that have the insulin gene. 5. When cells reproduce, the news cells will contain copies of the engineered plasmid. The foreign gene directs the cell to produce human insulin.
Suppose you transform bacteria with a recombinant DNA plasmid and by mistake grow the transformed cells without an antibiotic . What result would you observe?
If you grow transformed bacteria containing a recombinant DNA plasmid without an antibiotic, you would likely observe that only a small proportion of the cells that successfully took up the plasmid will survive, while the majority of non-transformed cells will also grow. However, the transformed cells may not express the gene of interest or provide any selective advantage, resulting in no significant difference in growth compared to the non-transformed cells. Over time, the population would likely consist mostly of non-transformed cells, as they do not require any selective pressure to thrive.
Bacterial cells which have been transformed with a plasmid are allowed to grow so that they the plasmids everytime they divide?
The transformed bacterial cells will replicate the plasmid along with their own genomic DNA each time they divide. This allows for amplification of the plasmid within the bacterial population. The plasmid can carry genes for antibiotic resistance, gene expression, or other functions that can be advantageous for the bacteria in certain conditions.
Why do scientists use the same enzyme to remove the insulin and cut the plasmid open?
Scientists use the same enzyme to remove insulin and cut the plasmid open for consistency and efficiency in genetic engineering processes. By utilizing the same restriction enzyme, they ensure that the sticky ends generated on both the insulin gene and the plasmid are complementary, facilitating the seamless insertion of the gene into the plasmid. This compatibility enhances the likelihood of successful ligation and subsequent expression of the insulin gene in host cells.
A population of cells carrying a desired plasmid is called a?
A population of cells carrying a desired plasmid is called a transformed population.
How does a human insulin genes become part of a plasmid?
1. Scientists remove plasmids, small rings of DNA, from bacterial cells. 2. An enzyme cuts open the plasmid DNA. The same enzyme removes the human insulin gene from its chromosome. 3. The human insulin gene attaches the open ends of the plasmid to form a closed ring. 4. Some bacterial cells take up the plasmids that have the insulin gene. 5. When cells reproduce, the news cells will contain copies of the engineered plasmid. The foreign gene directs the cell to produce human insulin.
How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the smae restriction enzyme use to cut out the hyman gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.
Suppose you transform bacteria with a recombinant DNA plasmid and by mistake grow the transformed cells without an antibiotic . What result would you observe?
If you grow transformed bacteria containing a recombinant DNA plasmid without an antibiotic, you would likely observe that only a small proportion of the cells that successfully took up the plasmid will survive, while the majority of non-transformed cells will also grow. However, the transformed cells may not express the gene of interest or provide any selective advantage, resulting in no significant difference in growth compared to the non-transformed cells. Over time, the population would likely consist mostly of non-transformed cells, as they do not require any selective pressure to thrive.
Bacterial cells which have been transformed with a plasmid are allowed to grow so that they the plasmids everytime they divide?
The transformed bacterial cells will replicate the plasmid along with their own genomic DNA each time they divide. This allows for amplification of the plasmid within the bacterial population. The plasmid can carry genes for antibiotic resistance, gene expression, or other functions that can be advantageous for the bacteria in certain conditions.
Why do scientists use the same enzyme to remove the insulin and cut the plasmid open?
Scientists use the same enzyme to remove insulin and cut the plasmid open for consistency and efficiency in genetic engineering processes. By utilizing the same restriction enzyme, they ensure that the sticky ends generated on both the insulin gene and the plasmid are complementary, facilitating the seamless insertion of the gene into the plasmid. This compatibility enhances the likelihood of successful ligation and subsequent expression of the insulin gene in host cells.
Why do the cells transformed with pUC18 and plasmid lux grow in the presence of ampicillin?
The pUC18 plasmid contains the ampicillin resistance gene (ampR) which confers resistance to ampicillin. The Lux operon on the plasmid allows for bioluminescence production and acts as a reporter gene. Therefore, transformed cells that harbor both plasmids can grow in the presence of ampicillin due to pUC18 and express bioluminescence due to the Lux operon.
Is the plasmid found in prokaryotic or eukaryotic cells?
The plasmid is found in prokaryotic cells.
What is the ampr gene?
The ampr gene encodes for the enzyme beta-lactamase, which confers resistance to ampicillin in bacteria. This gene is often used as a selectable marker in molecular biology experiments to identify transformed cells that have taken up a plasmid with the gene.
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
Cut the plasmid and foreign DNA with the same restriction enzyme to create complementary sticky ends. Mix the cut plasmid and foreign DNA together and ligate them using DNA ligase. Introduce the ligated plasmid into the bacterium using a method like transformation, where the bacterium uptakes the plasmid. Select for transformed bacteria using antibiotic resistance or another selectable marker on the plasmid.
The tumor inducing or Ti plasmid is useful in the genetic engineering of plants because it allows plant cells to be transformed even though plants do not contain plasmids?
True
Why is ampicillin added to the pound medium?
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means no plasmid in the bacterial host...
