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I. Transform bacteria with recombinant DNA molecule

II. Cut the plasmid DNA using restriction enzymes

III. Extract plasmid DNA from bacterial cells

IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments

V. Use ligase to seal plasmid DNA to nonplasmid DNA

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Why was the vector inserted into the bacterium?

The vector was inserted into the bacterium so as to artificially carry the foreign genetic materials into another cell.


What engineering involves inserting foreign dna into host dna to make recombinant DNA?

genetic


What is the process by which genes from one organism are transferred into DNA of another organism?

You would need to use Gene splicing to insert a foreign gene into an organism.


What would be the advantages of inserting into a plasmid gene with a highly visible phenotype?

Inserting a plasmid gene into the organism gives us three situation that one is the foreign cell may not pick up the plasmid the second chance is it is picked up may not expressed and in the third case it is expressed and therefore you can have the gene of interest. This is the one main advantage of studying the gene of interest by inserting a plasmid gene.


How does DNA technology transfer bacterial genes from one cell to another?

Gene transfer between cells generally consists of the following steps: 1. Isolating the gene of interest - Here, the gene which has to be transferred has to be isolated from the genome of the source (or host) organism. 2. Splicing the gene if interest into a plasmid. Splicing is a process wherein a foreign strand of DNA (the gene of interest) is inserted into a loop of DNA called a plasmid. The plasmid DNA is cut open to form a linear fragment. The gene of interest is then attached to the plasmid DNA. The plasmid DNA is converted back into the loop form with the help of an enzyme called DNA ligase. 3. Gene amplification: Here, the plasmid containing the gene of interest is amplified. Which means, many copies of the plasmid containing DNA are created through a process called the polymerase chain reaction. 4. Transfection: This is the final step wherein the plasmid containing DNA is inserted into the recipient organism. Sometimes the foreign DNA remains within the plasmid and is able to express protein. Other times, the gene of interest can be engineered to contain a sequence called the recombination sequence which will allow it to integrate (or join) the host genome through a process called homologous recombination. By the method described above, a foreign gene is removed from one organism and inserted into another. If the gene of interest is integrated into the host of the recipient organism, copies of it are made every time the host cells divide.

Related Questions

Why was the vector inserted into the bacterium?

The vector was inserted into the bacterium so as to artificially carry the foreign genetic materials into another cell.


Virus bacterium or toxins that the body regards as foreign is known as?

antigen


What engineering involves inserting foreign dna into host dna to make recombinant DNA?

genetic


Which includes the others plasmid foreign gene transformed bacterium recombinant DNA?

I have the same question


Compare the transformation of a bacterium cell with the transformation of a plant cell?

In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.


What is the process by which genes from one organism are transferred into DNA of another organism?

You would need to use Gene splicing to insert a foreign gene into an organism.


What results from inserting foreign DNA into an organism to produce a new gene combination?

Inserting foreign DNA into an organism can result in the production of a new gene combination known as a transgenic organism. This process can lead to the expression of new traits or proteins in the organism that were not originally present. These transgenic organisms can have applications in agriculture, medicine, and biotechnology.


What is the definition of interpolation?

The act of introducing or inserting anything, especially that which is spurious or foreign., That which is introduced or inserted, especially something foreign or spurious., The method or operation of finding from a few given terms of a series, as of numbers or observations, other intermediate terms in conformity with the law of the series.


Do tamper-resistant outlets require covers for safety purposes?

Yes, tamper-resistant outlets do not require additional covers for safety purposes as they are designed to prevent access to the electrical contacts by inserting foreign objects.


What is auto increment keys?

An auto-incremented key is a key (often used in a parent/foreign key constraint) that is generated automatically by a sequence number generator. The database provides the sequence generator, and generation/acquisition is wrapped in a transaction so that integrity can be preserved. Often, however, if a rollback is done, key values are lost, but this is not an issue in properly designed parent/foreign key relationships.


To what do antibodies respond?

Antibodies are secreted by a special group of white blood cells called lymphocytes. Each type of cell (including bacteria) has a unique protein on its cell membrane - an antigen. If a lymphocyte does not recognise a particular antigen, it will assume that the cell (or bacterium) is foreign and hostile. This is what antibodies respond to - the stimulus as the result of an unfamiliar cell. The antibodies attach to the antigens and kill the cell, or bacterium.


How does white blood cell kill a bacterium?

lymphocytes (a type of white blood cell) produce antibodies which will attach themselves to foreign particles. so a detected bacterium will have many antibodies surrounding it and stuck to it. antibodies which have attached themselves to the bacterium will send a signal to the phagocytes (another type of white blood cell) to come and 'eat'. so the phagocytes will engulf the bacterium, and secrete lysozyme (an enzyme which cuts up everything that comes into contact with it) to digest the bacterium, killing it. thank you for that :poopyloopy dark orbit.