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What is the term for a plasmid that contains a foreign gene?
Recombiant DNA
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
Cut the plasmid and foreign DNA with the same restriction enzyme to create complementary sticky ends. Mix the cut plasmid and foreign DNA together and ligate them using DNA ligase. Introduce the ligated plasmid into the bacterium using a method like transformation, where the bacterium uptakes the plasmid. Select for transformed bacteria using antibiotic resistance or another selectable marker on the plasmid.
How is the meaning recombine related to the production of recombinant DNA?
recombine joins together with means that the plasmid and the foreign dna join together to make recombinant dna
What engineering involves inserting foreign dna into host dna to make recombinant DNA?
genetic
Why was the vector inserted into the bacterium?
The vector was inserted into the bacterium so as to artificially carry the foreign genetic materials into another cell.
What is recombinant Plasmid?
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
A plasmid a foreign gene and recombinant DNA would be associated with what type of organism?
A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).
To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
When producing a recombinant plasmid, the plasmid and foreign DNA are cut with the same restriction enzyme(s) to generate complementary sticky ends for ligation. Using different restriction enzymes would create incompatible ends that cannot be ligated together effectively, making it difficult to form a functional recombinant plasmid.
Virus bacterium or toxins that the body regards as foreign is known as?
antigen
Why are bacterial cells useful in recombinant DNA technology?
Bacterial cells are useful in recombinant DNA technology because they can easily take up foreign DNA through a process called transformation. Once the foreign DNA is inserted into a bacterial cell, it can be replicated and amplified quickly. Bacteria are also easy to culture and manipulate in the laboratory, making them ideal for producing large quantities of recombinant proteins or DNA fragments.
How does salmonella E. work in recombinant DNA?
E. Coli is typically used as the host of recombinant DNA cloning. The process is as follows (simple version): DNA sample is cut use restriction sites, and a primer is loaded The cut DNA is place in a vector, example is a plasmid The plasmid is inserted into the host, example is E.Coli The E.Coli produces the DNA
What is the different between recombinant DNA and non-recombinant DNA?
Recombinant DNA is created by combining DNA from different sources, such as different species, through techniques like genetic engineering. Non-recombinant DNA refers to DNA that has not been modified in this way and only contains genetic material naturally found in an organism.
