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A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).

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What is the term for a plasmid that contains a foreign gene?

Recombiant DNA


To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?

When producing a recombinant plasmid, the plasmid and foreign DNA are cut with the same restriction enzyme(s) to generate complementary sticky ends for ligation. Using different restriction enzymes would create incompatible ends that cannot be ligated together effectively, making it difficult to form a functional recombinant plasmid.


What is recombinant Plasmid?

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.


What is the difference between the original plasmid and the recombinant plasmid?

Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.


What occurs first in the production of a recombinant plasmid?

In the production of a recombinant plasmid, the DNA of interest (insert) and the plasmid vector are both cut with restriction enzymes to create compatible ends. These cut fragments are then ligated together using DNA ligase to produce the recombinant plasmid.

Related Questions

What is the term for a plasmid that contains a foreign gene?

Recombiant DNA


How can a plasmid be engineered to include a piece of foreign DNA?

A plasmid can be engineered to include a piece of foreign DNA by using restriction enzymes to cut both the plasmid and the foreign DNA at specific sites. The two fragments are then ligated together using DNA ligase. The resulting recombinant plasmid can be introduced into a host organism for replication and expression of the foreign DNA.


To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?

When producing a recombinant plasmid, the plasmid and foreign DNA are cut with the same restriction enzyme(s) to generate complementary sticky ends for ligation. Using different restriction enzymes would create incompatible ends that cannot be ligated together effectively, making it difficult to form a functional recombinant plasmid.


What is recombinant Plasmid?

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.


Which includes the others plasmid foreign gene transformed bacterium recombinant DNA?

I have the same question


What is the difference between the original plasmid and the recombinant plasmid?

Orginal Plasmids are extra chromosomal genetic material present in eukaryotes and some prokaryotes.Recombinant plasmids contain a gene of intrest ie,individual gene carrying a specific function can be inserted in to a specific site on original plasmid in cell culture via transformation.So the recombinant plasmid contain both gene of intrest and native genes.


How is the meaning recombine related to the production of recombinant DNA?

recombine joins together with means that the plasmid and the foreign dna join together to make recombinant dna


How can bacteria be transformed with recombinant plasmid?

Bacteria can be transformed with recombinant plasmid by introducing the plasmid into the bacterial cell through a process called transformation. This allows the bacteria to take up the recombinant DNA from the plasmid and express the desired gene or trait encoded in the DNA.


What occurs first in the production of a recombinant plasmid?

In the production of a recombinant plasmid, the DNA of interest (insert) and the plasmid vector are both cut with restriction enzymes to create compatible ends. These cut fragments are then ligated together using DNA ligase to produce the recombinant plasmid.


Why is your plasmid considered recombinant DN?

A plasmid is considered recombinant DNA when it contains DNA sequences from multiple sources that have been artificially joined together using molecular cloning techniques. This can include the insertion of a gene of interest into the plasmid for expression in a host organism, or the addition of regulatory elements to control gene expression.


What does it meant by 'addition' in recombinant DNA?

In recombinant DNA technology, addition refers to the process of introducing a specific gene or DNA fragment into a plasmid or vector to create a genetically modified organism. This can involve inserting the gene of interest into the plasmid using restriction enzymes and ligases, allowing the gene to be expressed in the host organism.


When is a plasmid considered a recombinant plasmid?

A plasmid is considered recombinant when it contains DNA sequences from two different sources that have been artificially combined, often through genetic engineering techniques like restriction enzyme digestion and ligation. This results in a plasmid with modified or additional genetic material compared to its original form.