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It is to ensure that the spots of colouring are completely (or at least near that) separated into the different dyes that make them up. The further up the paper the solvent front is, the greater the separation of the spots. The different distances moved by the different dyes in proportion to the distance the solvent moved is known as the Rf value.
For more information, type: What is chromatography? It might help.
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Why must the chromatogram be removed before the solvent travels the entire length of the paper?
So that you can measure how far it traveled.
What is the function of pump in HPLC?
A high-performance liquid chromatography, or HPLC, refers to a technique in analytic chemistry that is used to separate the components in a mixture. The pump in HPLC passes a pressurized liquid solvent that contains the sample mixture through a column filled with a solid adsorbent material.
Why do you need to cover the beaker with a lid during paper chromatography?
During the development of a chromatogram, it is better to cover it with a beaker to prevent evaporation, which will affect the movement of the solute and solvent system. This will ensure that the chromatogram will develop properly.
What does it mean if the spot is in the solvent front in chromatography?
it means that the solvent dissolves the solute.
What is the function of the mobile phase for developing a chromatogram?
stationary phase is the solid called the filter paper and mobile phase is the liquid or organic solvent present in the developing tank or beaker by ayesha zulfiqar
Why the beaker covered during the development of chromatogram?
During the development of a chromatogram, it is better to cover it with a beaker to prevent evaporation, which will affect the movement of the solute and solvent system. This will ensure that the chromatogram will develop properly.
You developed your chromatogram too long and you can't find the solvent front describe and explian the possible effect on your results of the error?
If this happened you would not be able to calculate the Rf value. If you can't find the Rf value you will not be able to tell if the solvent is unknown.
3 Why do the pigments become separated during the development of the chromatogram?
The chromatogram, or chromatography paper has solvent on it. Each pigment will separate itself from the others due to the relative solubility of that particular pigment.
Why must the chromatogram be removed before the solvent travels the entire length of the paper?
So that you can measure how far it traveled.
What is the function of pump in HPLC?
A high-performance liquid chromatography, or HPLC, refers to a technique in analytic chemistry that is used to separate the components in a mixture. The pump in HPLC passes a pressurized liquid solvent that contains the sample mixture through a column filled with a solid adsorbent material.
What is paper chromatograph?
Ascending paper chromatography is where a chromatogram rises, or ascends. The paper moves up because the solvent moves it in an upward direction.
What is ascending paper chromatograph?
Ascending paper chromatography is where a chromatogram rises, or ascends. The paper moves up because the solvent moves it in an upward direction.
Why do you need to cover the beaker with a lid during paper chromatography?
During the development of a chromatogram, it is better to cover it with a beaker to prevent evaporation, which will affect the movement of the solute and solvent system. This will ensure that the chromatogram will develop properly.
What are largest and smallest Rf values?
The largest Rf value is 1, which occurs when the compound remains at the origin of the chromatogram and does not move at all. The smallest Rf value is 0, which happens when the compound moves with the solvent front and does not adhere to the stationary phase.
What does it mean if the spot is in the solvent front in chromatography?
it means that the solvent dissolves the solute.
What is the function of the mobile phase for developing a chromatogram?
stationary phase is the solid called the filter paper and mobile phase is the liquid or organic solvent present in the developing tank or beaker by ayesha zulfiqar
Where on the filter paper do you expect the larger molecules to be located in the final chromatogram?
Towards the centre of the chromatogram. The smaller molecules are more mobile and appear towards the outside of the chromatogram.
