The Kirby-Bauer methods is a standardized system that takes all variables into consideration:
The recommended medium in this test is Mueller-Hinton II agar. Its pH should be between 7.2 and 7.4, and it should be poured to a uniform thickness of 4mm in the petri plate. This requires 60 ml in a 150 mm plate and 25 ml in a 100 mm plate. For certain fastidious organisms, 5% defibrinated sheep blood is added to the medium.
Inoculation of the surface of the medium is made with a cotton swab from a broth culture. High-potency disks are used that may be placed on the agar with a mechanical dispenser or sterile forceps. To secure the disks to the medium, it is necessary to press them down ont the agar.
After 16 to 18 hours incubation, the plates are examined and the diameters of the zones are measured to the nearest millimeter. In this case, we are measuring what is called the "zone of inhibition" or where no growth occurs. And lastly, record your results. The zone diameter is compared to a standard table for that drug and concentration, and the organism is reported as sensitive, intermediate, or resistant. For a drug with poor solubility, however, the zone of inhibition indicating that the microbe is sensitive will usually be smaller than for another drug that is more soluble and has diffused more widely. Results obtained by the disk-diffusion method (AKA Kirby-Bauer test) are often inadequate for many clinical purposes. However, the test is simple and inexpensive and is most often used when more sophisticated laboratory facilities are not available.
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method
In CTAB method,SEVAG is used to breakdown the tissues in the extracted leaves.While in dellaporta method,the SDS and POTASSIUM ACETATE are used.In CTAB method BLUECAP/TEST TUBES are used,while in dellaporta method the EMPENDORFS are mostly used.ICE COLD ETHANOL is used mostly in the CTAB method for resuspension,while in dellaporta method ISOPROPANOL is used.
Sequencing DNA (Sanger Sequencing Method).
is the scientific method cylic or linear?
historical method observational method expermental method
How is the method superior to the payback method
How is the method superior to the payback method
Declaring a method is when you code for what the method will perform. When you call a method, you are using the method you have written in another part of the program, (or inside the method if it is recursive).
cone method,deep hole method,slide hole method,center hole method,trench method,slit method
You can not increase a "Method". A method is a way of doing something. You could change your method, improve your method, simplify tour method, but NOT "increase" it.
Some methods of teaching commerce include lectures, case studies, simulations, group discussions, and experiential learning opportunities. Teachers might also incorporate real-world examples, guest speakers, and hands-on projects to help students gain a practical understanding of commerce concepts. Providing opportunities for students to apply their knowledge in real-life scenarios can enhance their learning experience.
No, the calendar method is a NATURAL method.
What is your method of study? I like your Method.
deductive method inductive method
Solfege method.
No. It is a non-invasive method