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What is the Lactococcus lactis ssp lactis oxidase test result?
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∙ 13y ago
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What is the test that separates enterococcus faecalis and lactococcus lactis?
To diagnose Enterococcus faecalis: (Facultative anaerobic) First, do the Gram stain: Gram positive cocci in chains catalase test: negative PYR disc: positive
Are there limitations to the oxidase test?
There are none.
Give explanation why the Oxidase test identifies the presence of cytochrome c oxidase and not the other electron carriers?
An oxidase test relies on a reagent that will change colors when it is oxidized. The reagent used in the test only reacts to cytochrome c oxidase by acting in place of oxygen to receive electrons from the cytochrome.
Why is it advisable to include a known oxidase-positive control in any test of an unknown organism?
It's advisable so that the oxidase test determines whether or not an organism has cytochrome oxidase in its electron transport chain.
What is the function of the test reagent in the oxidase test?
The ability of bacteria to produce cytochrome oxidase can be determined by the addition of the test reagent p-aminodimethylaniline oxalate to colonies grown on a plate medium.
What are the results of a catalase test on Lactococcus lactis?
E.coli shows positive result for catalase test..staphylococcus aureus and serratia marcescens too..pseudomonas and klebsiella does not give any vigorous bubbling therefore, it shows negative result
What is the test that separates enterococcus faecalis and lactococcus lactis?
To diagnose Enterococcus faecalis: (Facultative anaerobic) First, do the Gram stain: Gram positive cocci in chains catalase test: negative PYR disc: positive
Why should nichrome or iron-containing inoculating devices not be used in the oxidase test?
It can result in a false positive result.
Is the media for oxidase test selective or differential?
media used for oxidase test is non selective media
Are all aerobic bacteria oxidase positive?
yes, all aerobic cells are oxidase positive. The oxidase test is testing for the presence of cytochrome oxidase [by reducing it with an artificial substrate which turns purple when oxidised]. In aerobic cells cytochrome oxidase's normal role is in the electron transport chain, passing electrons from cytochrome c to oxygen. hence if the cell is aerobic then oxygen is terminal electron acceptor in the cells ETC and thus it will give a positive result in the oxidase test.
Are there limitations to the oxidase test?
There are none.
Give explanation why the Oxidase test identifies the presence of cytochrome c oxidase and not the other electron carriers?
An oxidase test relies on a reagent that will change colors when it is oxidized. The reagent used in the test only reacts to cytochrome c oxidase by acting in place of oxygen to receive electrons from the cytochrome.
Why is it advisable to include an oxidase-positive control organism in any test of an unknown organism?
It's advisable so that the oxidase test determines whether or not an organism has cytochrome oxidase in its electron transport chain.
Why is it advisable to include a known oxidase-positive control in any test of an unknown organism?
It's advisable so that the oxidase test determines whether or not an organism has cytochrome oxidase in its electron transport chain.
Why is It advisable to include an oxidase positive control organism in any test of an unknown organism?
It's advisable so that the oxidase test determines whether or not an organism has cytochrome oxidase in its electron transport chain.
Does E. coli test positive for oxidase?
no
What is another test used for the identification of Staphylococcus aureus?
oxidase test.
