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What is Urease test results for bacillus cereus?
Bacillus cereus is positive for the urease test, meaning it can break down urea to produce ammonia and increase the pH of the media. This can be observed by a color change in the media from yellow to pink or magenta.
What is the hydrogen sulfide test used on Bacillus subtilis?
H2S (hydrogen sulfide) Test: This test is used to check for Enteric gram negative bacilli by checking for the enzyme thiosulfate reductase which is produced by various intestinal microorganisms. So if bacillus subtilis can produce this enzyme then this test will give you a pos. (black in color) result. Yeah, no kidding. I'm pretty sure the question was what the result of it, or they wouldn't specify that it was b. subtilis. and it is positive for H2S production.
Is Bacillus subtilis positive for lactose fermentation?
No, Bacillus subtilis is not capable of fermenting lactose as it lacks the enzymes required for lactose metabolism. It is able to utilize other carbohydrates such as glucose and maltose for energy production.
Is Alcaligenes faecalis oxidase positive?
yes and no, sometimes it comes out positive and sometimes it comes out negitive
What are the biochemical tests for identification of bacillus subtilis?
The Kirby-Bauer antibiotic test is a general test that determines if an organism is resistant or susceptible to different antibiotics. Many different types of antibiotics can be used but some of the common ones are penicillin, tetracycline, chloramphenicol, and streptomycin.
Does bacillus subtilis have a positive reaction for ONPG Test?
yes it have positive reaction for ONPG test
What is the result of citrate test for bacillus subtilis?
blue/positive
Does bacilus subtilis test negative for coagulase test?
Yes, Bacillus subtilis is negative for the coagulase test. Coagulase is an enzyme produced by some bacteria (such as Staphylococcus aureus) that causes blood plasma to clot, resulting in a positive test. Bacillus subtilis does not produce coagulase, so it will not clot the plasma and will have a negative test result.
What is Urease test results for bacillus cereus?
Bacillus cereus is positive for the urease test, meaning it can break down urea to produce ammonia and increase the pH of the media. This can be observed by a color change in the media from yellow to pink or magenta.
What are the results for litmus milk test on Bacilus subtilis?
Bacillus subtilis will typically produce an alkaline reaction in litmus milk, turning it pink or purple. This is due to the bacterium's ability to break down lactose and produce ammonia. Additionally, Bacillus subtilis may also cause coagulation of the milk proteins, resulting in a solid clot formation.
What is the hydrogen sulfide test used on Bacillus subtilis?
H2S (hydrogen sulfide) Test: This test is used to check for Enteric gram negative bacilli by checking for the enzyme thiosulfate reductase which is produced by various intestinal microorganisms. So if bacillus subtilis can produce this enzyme then this test will give you a pos. (black in color) result. Yeah, no kidding. I'm pretty sure the question was what the result of it, or they wouldn't specify that it was b. subtilis. and it is positive for H2S production.
Is Bacillus subtilis positive for lactose fermentation?
No, Bacillus subtilis is not capable of fermenting lactose as it lacks the enzymes required for lactose metabolism. It is able to utilize other carbohydrates such as glucose and maltose for energy production.
Why does the oxidase reagent need to be fresh?
The oxidase reagent needs to be fresh because it contains the enzyme cytochrome c oxidase, which can degrade over time, leading to false-negative results if it is not active. Using fresh reagent ensures the accuracy of the test results.
Is Alcaligenes faecalis oxidase positive?
yes and no, sometimes it comes out positive and sometimes it comes out negitive
Why should nichrome or iron-containing inoculating devices not be used in the oxidase test?
Nichrome or iron-containing inoculating devices should not be used in the oxidase test because they can introduce false-positive results. The metal ions from these devices can react with the reagents used in the oxidase test, leading to the appearance of a positive result even if the organism being tested is actually negative for oxidase activity. It is important to use a non-reactive, disposable inoculating loop or swab to avoid interference with the test results.
What is the difference between bacillus coccus and spirillum?
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
What are the biochemical tests for identification of bacillus subtilis?
The Kirby-Bauer antibiotic test is a general test that determines if an organism is resistant or susceptible to different antibiotics. Many different types of antibiotics can be used but some of the common ones are penicillin, tetracycline, chloramphenicol, and streptomycin.
