Bacillus cereus is oxidase negative -
Most bacteria in Tribe V are able to utilize Citrate, with the exception of Hafnia and Pantoea (+/-). The 2 most common Klebsiella (oxytoca and pneumoniae), can utilize citrate, so should be citrate positive.
Pseudomonas aeruginosa typically shows negative results on a urease test, as it does not produce urease enzyme. This means it does not break down urea into ammonia and carbon dioxide, resulting in no change in pH and no color change in the test medium.
When determining the ability of a bacteria to degrade urea by means of the enzyme urease. To diagnose H. pylori and identification of the genus Proteus, Providentia, Kelbsiella, and Morganella by their fast urease activity.
Staph gives both MR and VP positive. MR is used to detect acid production and VP for alkaline or neutral products. Staph first converts into acidic products and has ability to convert those acidic products into some neutral/basic products side by side. Hence it gives both positive results.
Bacillus subtilis is positive for the oxidase test. This means that it has the enzyme cytochrome c oxidase, which is needed for the bacterium to produce energy through aerobic respiration. The positive result is indicated by the appearance of a color change (usually dark purple) on the test strip after adding the reagent.
bacillus megatarium test positive for sucrose where creus doesnt
Bacillus cereus is generally susceptible to erythromycin. However, it's important to note that antibiotic susceptibility can vary, so it's recommended to perform a susceptibility test to confirm the effectiveness of erythromycin against a specific strain of Bacillus cereus.
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
The easiest way to distinguish one from the other is by performing an amylase test. Bacillus cereus will test positive by displaying a clear zone around the bacteria. Clostridium sporogenes will test negative because it does not produce the exoenzyme amylase. Another way is by performing a catalase test. Bacillus will test positive because it's an aerobe and Clostridium will test negative because it's anaerobic.
The B.cereus has a positive citrate test and a negative Voges Proskauer.
Most bacteria in Tribe V are able to utilize Citrate, with the exception of Hafnia and Pantoea (+/-). The 2 most common Klebsiella (oxytoca and pneumoniae), can utilize citrate, so should be citrate positive.
Pseudomonas aeruginosa typically shows negative results on a urease test, as it does not produce urease enzyme. This means it does not break down urea into ammonia and carbon dioxide, resulting in no change in pH and no color change in the test medium.
Salmonella paratyphi typically shows a negative result in the urease test. This means that it does not produce the enzyme urease, which is responsible for hydrolyzing urea into ammonia and carbon dioxide. As a result, there is no color change in the medium used for the test, indicating the absence of urease activity.
When determining the ability of a bacteria to degrade urea by means of the enzyme urease. To diagnose H. pylori and identification of the genus Proteus, Providentia, Kelbsiella, and Morganella by their fast urease activity.
Staph gives both MR and VP positive. MR is used to detect acid production and VP for alkaline or neutral products. Staph first converts into acidic products and has ability to convert those acidic products into some neutral/basic products side by side. Hence it gives both positive results.
Because M.tuberculosis produces urease. thus indicating a person has the bacteria can cause tuberculosis.
The medium used for the rapid urease test for H. pylori is called urea agar or urea broth. It contains urea as a substrate and a pH indicator to detect the presence of the enzyme urease produced by H. pylori.