Staph gives both MR and VP positive. MR is used to detect acid production and VP for alkaline or neutral products. Staph first converts into acidic products and has ability to convert those acidic products into some neutral/basic products side by side. Hence it gives both positive results.
its obvious!! methyl red test tells us if the bacteria is using mixed acid fermentation process. if it is following it will show +ve. vp test is used to detect whether acetotin is produced or not.if bacteria is using mixed acid fermentation process acetotin will not be produced. so our bacteria can be either mr +ve or vp -ve. or it can be both negative....it cant be both +ve for further doubts ping me at sandeepdsensation@gmail.com
Most bacteria in Tribe V are able to utilize Citrate, with the exception of Hafnia and Pantoea (+/-). The 2 most common Klebsiella (oxytoca and pneumoniae), can utilize citrate, so should be citrate positive.
Bacillus brevis typically shows beta-hemolysis on blood agar plates, where the red blood cells are completely lysed and a clear zone surrounds the colonies. This indicates that the bacterium produces hemolysins that lyse red blood cells.
The Voges-Proskauer test is used to detect acetoin production, which is a metabolic byproduct of certain bacteria such as Enterobacter, Serratia, and Hafnia. These organisms undergo specific metabolic pathways that produce acetoin, leading to a positive Voges-Proskauer test result.
It is abbreviated MR/VP. MR stands for methyl red, and VP stands for Voges-Proskauer. It is used to identify bacteria producing stable acids by mechanisms of mixed acid fermentation and glucose. I found this on wikipedia.com.
Negative Negative Negative Negative
The B.cereus has a positive citrate test and a negative Voges Proskauer.
An organism can not test positive for both an MR test and a V - P test. The MR test is an abbreviation for Methyl Red and it tests for E coli The V - P is an abbreviation for Vogue Proskauer and it tests for enterobacter and klebsiella.
Shaking the MR-VP culture is often done to aerate the medium, improve oxygen transfer, and ensure proper growth conditions for microorganisms. This action helps enhance bacterial metabolism, particularly in the context of sugar fermentation in the MR-VP test.
its obvious!! methyl red test tells us if the bacteria is using mixed acid fermentation process. if it is following it will show +ve. vp test is used to detect whether acetotin is produced or not.if bacteria is using mixed acid fermentation process acetotin will not be produced. so our bacteria can be either mr +ve or vp -ve. or it can be both negative....it cant be both +ve for further doubts ping me at sandeepdsensation@gmail.com
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
Most bacteria in Tribe V are able to utilize Citrate, with the exception of Hafnia and Pantoea (+/-). The 2 most common Klebsiella (oxytoca and pneumoniae), can utilize citrate, so should be citrate positive.
The Voges-Proskauer (VP) test detects the presence of acetoin, an intermediate product in the fermentation of glucose to 2,3-butanediol. In the VP test, a culture is first grown in a medium containing glucose and then reagents are added to detect the presence of acetoin. A positive VP test is indicated by a red color development after the addition of alpha-naphthol and KOH reagents.
A VP (Voges-Proskauer) test for Bacillus is used to determine the ability of the bacteria to produce acetoin from glucose fermentation. A positive VP test result is indicated by the development of a red color after the addition of Barritt's reagents A and B, showing the presence of acetoin.
Bacillus megaterium
David White but in 2008 will change to Mr Muthoo
I was hoping to find an answer here.