Staph gives both MR and VP positive. MR is used to detect acid production and VP for alkaline or neutral products. Staph first converts into acidic products and has ability to convert those acidic products into some neutral/basic products side by side. Hence it gives both positive results.
MR-VP testing is not necessary for B. cereus. A FTM test for (+) would be B. cereus and FTM test for (-) would be B. megaterium or B. subtilis. MR-VP is used to determine B. megaterium from B. subtilis. If MR is (+) would be B. subtilis, if VP (-) would be B. megaterium. --- Abstracted from Microbiology Laboratory Theory & Application (Brief Edition), pg. 476.
MR-VP tests to not conclude anything for Gram + cocci, and should only be used to identify Gram - rods. Use Catalase as a positive control test for the presence of Gram + cocci.
very good chance it is VP negative
Bacillus Brevis is alfa hemolytic for blood, it does not hydrolyses starch very well, nor urea and is VP negative (That means is MR positive).
its obvious!! methyl red test tells us if the bacteria is using mixed acid fermentation process. if it is following it will show +ve. vp test is used to detect whether acetotin is produced or not.if bacteria is using mixed acid fermentation process acetotin will not be produced. so our bacteria can be either mr +ve or vp -ve. or it can be both negative....it cant be both +ve for further doubts ping me at sandeepdsensation@gmail.com
It is abbreviated MR/VP. MR stands for methyl red, and VP stands for Voges-Proskauer. It is used to identify bacteria producing stable acids by mechanisms of mixed acid fermentation and glucose. I found this on wikipedia.com.
MR n VP tests always made wid each other becoz da media used for performing da test is divided into two parts n then used simaltaneously....
Klebsiella sp.
Negative Negative Negative Negative
The B.cereus has a positive citrate test and a negative Voges Proskauer.
An organism can not test positive for both an MR test and a V - P test. The MR test is an abbreviation for Methyl Red and it tests for E coli The V - P is an abbreviation for Vogue Proskauer and it tests for enterobacter and klebsiella.
Bacillus Brevis is alfa hemolytic for blood, it does not hydrolyses starch very well, nor urea and is VP negative (That means is MR positive).
The MR-VP culture is shaken to oxygenate the culture.
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
its obvious!! methyl red test tells us if the bacteria is using mixed acid fermentation process. if it is following it will show +ve. vp test is used to detect whether acetotin is produced or not.if bacteria is using mixed acid fermentation process acetotin will not be produced. so our bacteria can be either mr +ve or vp -ve. or it can be both negative....it cant be both +ve for further doubts ping me at sandeepdsensation@gmail.com
negative!
It means that the bacterial organism uses the butylene glycol pathway; producing acetoin (acetylmethylcarbinol). This specific compound is produced only by bacteria that utilize this pathway. Usually (but not always) a positive Voges-Proskauer (VP) test indicates the organism is negative for the methyl red (MR) test. Both the MR and VP tests are usually done using the same culture; after dividing it into two equal volumes.
reaction of voges-proskauer test
It is abbreviated MR/VP. MR stands for methyl red, and VP stands for Voges-Proskauer. It is used to identify bacteria producing stable acids by mechanisms of mixed acid fermentation and glucose. I found this on wikipedia.com.
Bacillus megaterium