Bacillus Brevis is alfa hemolytic for blood, it does not hydrolyses starch very well, nor urea and is VP negative (That means is MR positive).
It is NEGATIVE
Bacillus cereus is oxidase negative -
Bacillus and Clostridium are both Gram positive, spore forming, bacilli (rods).Bacillus sp. are aerobic.Clostridium sp. are anaerobic.After performing a catalase activity test, Clostridium will be negative and Bacillus will be positive for catalase activity.
No, it is negative.B. subtilis on EMB agar:http://www.microbelibrary.org/microbelibrary/files/ccImages/Articleimages/Atlas_EMB/Bacillus-subtilis_EMB_fig11.jpg
H2S (hydrogen sulfide) Test: This test is used to check for Enteric gram negative bacilli by checking for the enzyme thiosulfate reductase which is produced by various intestinal microorganisms. So if bacillus subtilis can produce this enzyme then this test will give you a pos. (black in color) result. Yeah, no kidding. I'm pretty sure the question was what the result of it, or they wouldn't specify that it was b. subtilis. and it is positive for H2S production.
It is NEGATIVE
Bacillus cereus is oxidase negative -
bacillus megatarium test positive for sucrose where creus doesnt
alpha
Alpha hemolysis (α-hemolysis) means that the bacterial enzymes only partially break down the blood cells. This results in the media showing a yellowish/greenish/brownish discoloration (like a bruise) around the colonies, indicating incomplete hemolysis.How to Interpret Beta Hemolysis on Blood Agar: Test for Strep Throat - Identifying B-hemolytic Streptococcus
negative!
red blodd cell hemolysis
Most Bacillus species are Catalase positive.
blue/positive
yes it have positive reaction for ONPG test
Bacillus and Clostridium are both Gram positive, spore forming, bacilli (rods).Bacillus sp. are aerobic.Clostridium sp. are anaerobic.After performing a catalase activity test, Clostridium will be negative and Bacillus will be positive for catalase activity.
The easiest way to distinguish one from the other is by performing an amylase test. Bacillus cereus will test positive by displaying a clear zone around the bacteria. Clostridium sporogenes will test negative because it does not produce the exoenzyme amylase. Another way is by performing a catalase test. Bacillus will test positive because it's an aerobe and Clostridium will test negative because it's anaerobic.