blue/positive
yes it have positive reaction for ONPG test
Yes, Bacillus subtilis is negative for the coagulase test. Coagulase is an enzyme produced by some bacteria (such as Staphylococcus aureus) that causes blood plasma to clot, resulting in a positive test. Bacillus subtilis does not produce coagulase, so it will not clot the plasma and will have a negative test result.
The result is the initial green color of the Simmons Citrate Agar turning blue; generating a positive indication that the organism does indeed utilize citrate as the sole source of carbon and energy.
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
If Bacillus subtilis is inoculated into litmus milk, it will likely grow and metabolize lactose, forming acid as a byproduct. This metabolism will cause the litmus milk to turn pink or purple as the pH decreases due to the production of acid. The milk may also show coagulation due to acid production.
yes it have positive reaction for ONPG test
Yes, Bacillus subtilis is negative for the coagulase test. Coagulase is an enzyme produced by some bacteria (such as Staphylococcus aureus) that causes blood plasma to clot, resulting in a positive test. Bacillus subtilis does not produce coagulase, so it will not clot the plasma and will have a negative test result.
Most bacteria in Tribe V are able to utilize Citrate, with the exception of Hafnia and Pantoea (+/-). The 2 most common Klebsiella (oxytoca and pneumoniae), can utilize citrate, so should be citrate positive.
H2S (hydrogen sulfide) Test: This test is used to check for Enteric gram negative bacilli by checking for the enzyme thiosulfate reductase which is produced by various intestinal microorganisms. So if bacillus subtilis can produce this enzyme then this test will give you a pos. (black in color) result. Yeah, no kidding. I'm pretty sure the question was what the result of it, or they wouldn't specify that it was b. subtilis. and it is positive for H2S production.
The result is the initial green color of the Simmons Citrate Agar turning blue; generating a positive indication that the organism does indeed utilize citrate as the sole source of carbon and energy.
Bacillus subtilis is positive for the oxidase test. This means that it has the enzyme cytochrome c oxidase, which is needed for the bacterium to produce energy through aerobic respiration. The positive result is indicated by the appearance of a color change (usually dark purple) on the test strip after adding the reagent.
The B.cereus has a positive citrate test and a negative Voges Proskauer.
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
No, Bacillus subtilis is not capable of fermenting lactose as it lacks the enzymes required for lactose metabolism. It is able to utilize other carbohydrates such as glucose and maltose for energy production.
Bacillus subtilis will typically produce an alkaline reaction in litmus milk, turning it pink or purple. This is due to the bacterium's ability to break down lactose and produce ammonia. Additionally, Bacillus subtilis may also cause coagulation of the milk proteins, resulting in a solid clot formation.
Pseudomonas aeruginosa typically demonstrates a negative result on the citrate test, as it is unable to utilize citrate as its sole carbon source for growth. This bacterium lacks the enzyme citrate-permease needed to transport citrate into the cell for metabolism, leading to a lack of growth on citrate agar and a lack of color change from green to blue.
If Bacillus subtilis is inoculated into litmus milk, it will likely grow and metabolize lactose, forming acid as a byproduct. This metabolism will cause the litmus milk to turn pink or purple as the pH decreases due to the production of acid. The milk may also show coagulation due to acid production.