citrate result is positive in case of pseudomonas aeroginosa. slant and butt both will be positive
negative
positive
in the absence of glucose or lactose , some bacteria are able to use citrate as only source of carcon. alkaline pH change the bromothymol blue indicator from green to blue. pseudomonas can not ferment the glucose and the lactose so it use the citrate as carbon source.
Staphylococcus Epidermidis does return a gram positive result on the citrate lab test. Gram stains will produce these results on slides.
Citrate Permease is an enzyme that is responsible for transporting citrate inside the cell. Some bacteria have this enzyme while others do not. You can test for the presence of citrate permease by performing a Simmon's Citrate biochemical test.
The positive test control organism for citrate utilization test is Klebsiella pneumoniae
positive
this test is used to identify bacteria which use citrate as sole source of carbon e.g klebsila, pseudomonas aerogenosa
This is a bit tricky. Both Proteus vulgaris and Pseudomonas aeruginosa are Gram-negative and rod-shaped. According to Bergey's Manual of Systematic Bacteriology, Proteus vulgaris tests positive for sulfur reduction, urease production, tryptophan deaminase production, and indole production. Pseudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria.An even easier way to test this is the gelatinase test.Or, slant the bacteria on a Triple Sugar Iron slant or place them in a SIM agar deep. P. vulgaris tests positive for Hydrogen Sulfide production, changing the media to a black color around the organism, while P. aeruginosa does not produce Hydrogen Sulfide.
blue/positive
The result is the initial green color of the Simmons Citrate Agar turning blue; generating a positive indication that the organism does indeed utilize citrate as the sole source of carbon and energy.
in the absence of glucose or lactose , some bacteria are able to use citrate as only source of carcon. alkaline pH change the bromothymol blue indicator from green to blue. pseudomonas can not ferment the glucose and the lactose so it use the citrate as carbon source.
I couldn't tell you what were the odds, but I hope this helps: You could be killing your oxidase activity if your organism was no longer viable. When identifying organisms you should always use fresh cultures. Regardless, some strains of Pseudomonas aeruginosa can test oxidase negative. a TSI slant would confirm if you were dealing with a presumptive pseudomonad or an enteric. Enterics will have a strong acid reaction (because they ferment) and Pseudomonas would have no acid (or a very weak acid) reaction. Happy streaking!
Members of the genus Pseudomonas display the following defining characteristics: • Rod shaped •Gram-negative • One or more polar flagella, providing motility • Aerobic, although some species have been found to be facultative anaerobes (e.g. P. aeruginosa) •Non–spore forming • Positive catalase test
Staphylococcus Epidermidis does return a gram positive result on the citrate lab test. Gram stains will produce these results on slides.
Enterobacteriaceae are nitrate +. Don't know about Pseudomonas, though.
Based on my own test, Shigella flexneri tests negative for the citrate test.
Citrate Permease is an enzyme that is responsible for transporting citrate inside the cell. Some bacteria have this enzyme while others do not. You can test for the presence of citrate permease by performing a Simmon's Citrate biochemical test.