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Phagocytosis
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∙ 11y ago
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What is primary and secondary antibody?
primary antibody is what binds to the specific gene that you are interested in looking at; i.e. primary is rabbit-antibody bind to its proper epitope. and this is usually unconjugated with no label. the secondary antibody is conjugated with some type of label, i.e., you will be able to see if your gene is being expressed. i.e., if primary from a rabbit, want goat-anti-rabbit, this way it can bind to the primary antibody.
Is complement fixation an chief antibody mediated mechanism?
Yes
What is the effect of not including the antigen or the primary antibody in the ELISA reaction?
Not including the antigen will prevent the primary antibody from binding to it which will disrupt the results of the ELISA. Not including the primary antibody will prevent the secondary antibody from binding it, which will again negatively affect the results of the ELISA. All components are necessary to get an accurate ELISA.
what antibody use in western blot technique?
Generally there are two antibodies used. Primary antibody which can bind specifically to the protein of interest. And a secondary antibody coupled with a detection system such as HRP that would bind the primary antibody and signals the presence of protein of interest.
How specific protein can be detected in western blot?
Specific proetins can be detected by its specific mono clonal antibody. Primary antibodies specifically binds to the proetins on the membrane. Secondary antibody interact with primary antibody and signals its presence by chemiluminescence.
What is the first antibody made in response to an antigen?
IgM is the antibody first secreted during primary response
What is mechanism of action eclipta Alba as hepatoprotective?
What is mechanism of action eclipta Alba as hepatoprotective?
What is the Mechanism of action of tiemonium methylsulphate?
+mechanism of action of tiemonium methylsulfate
What is the Primary mechanism of homeostatic regulation?
The body's primary mechanism of homeostatic regulation is negative feedback. This mechanism recognizes the problem, identifies the correction, and changes the variable.
What is the body's primary mechanism of homeostatic regulation?
The body's primary mechanism of homeostatic regulation is negative feedback. This mechanism recognizes the problem, identifies the correction, and changes the variable.
What is flomax's diuretic mechanism of action action?
mod of action tamsulosin
The primary mechanism of natural selection is?
Evolution
