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What is the advantage of reading the absorbance rather than the percent transmittance on the spectrophotometer?
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∙ 14y ago
because, aliens
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∙ 10y ago
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Explain in detain how the spectrophotometer works?
The spectrophotometer is a complex instrument used in measuring the absorbance of biomolecules within the ultraviolet and visible light spectrum, similar to the one found in the laboratory. It is a conglomerate of light sources, wavelength selectors, optical systems, sample chambers, photodetectors, and meters functioning together to perform a specific task - to measure the absorbance of a sample. It works by a light passing through a solution, the higher the M concentration of the solution the more light is absorbed. The percent of transmittance will help analysis the M concentration
What happens to light that strikes clear plastic?
A common technique in chemistry is spectroscopic analysis. This is where light is passed through a solution to determine its composition. The spectrometer will give a reading of absorbance or percent transmittance. The absorbance tells you how much light is absorbed, while the percent transmittance tells you what percentage of the light passed through and was not absorbed. If when you say clear you mean "colorless," all the light will pass through. The plastic is most likely not entirely colorless so only a small portion of the light will be absorbed, and it will have a high percent transmittance value.
Why is absorbance plotted instead of percent transmission when following bacterial growth?
Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.
What reasons can you give for the difference in the percentage of transmittance between the live chlorplats that were incubated in the light and those that were kept in the dark?
The percent transmittance grew to steadily higher numbers as the experiment progressed because the light reaction was able to occur. However, the dark cuvettes had stable levels of transmittance because light is necessary to excite electrons, which, in turn, reduces the DPIP.
Light transmittance can be more then 100 percent?
logically no because if it is a yes, then the light reaching the detector is greater than the light which was produced by the machine in the first place. But you may get transmittance greater than 100 because some parameters of your experiment may not be right.
How can you figure out Absorbance without percent transmittance?
A=logIo/I
What is the relationship between absorbance and transmittance?
Absorbance = -log (percent transmittance/100)
Explain in detain how the spectrophotometer works?
The spectrophotometer is a complex instrument used in measuring the absorbance of biomolecules within the ultraviolet and visible light spectrum, similar to the one found in the laboratory. It is a conglomerate of light sources, wavelength selectors, optical systems, sample chambers, photodetectors, and meters functioning together to perform a specific task - to measure the absorbance of a sample. It works by a light passing through a solution, the higher the M concentration of the solution the more light is absorbed. The percent of transmittance will help analysis the M concentration
What happens to light that strikes clear plastic?
A common technique in chemistry is spectroscopic analysis. This is where light is passed through a solution to determine its composition. The spectrometer will give a reading of absorbance or percent transmittance. The absorbance tells you how much light is absorbed, while the percent transmittance tells you what percentage of the light passed through and was not absorbed. If when you say clear you mean "colorless," all the light will pass through. The plastic is most likely not entirely colorless so only a small portion of the light will be absorbed, and it will have a high percent transmittance value.
How do you convert percent transmittance to mgL?
how do you convert percent transmittance to mg/l
Why do chemists prefer to read the absorbance rather than the percent transmittance of lght when analyzing a sample having a visible color?
Because when you graph a molar concentration vs. absorbance graph, the graph is linear, making the graph easier to read.
Why is absorbance plotted instead of percent transmission when following bacterial growth?
Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.
What reasons can you give for the difference in the percentage of transmittance between the live chlorplats that were incubated in the light and those that were kept in the dark?
The percent transmittance grew to steadily higher numbers as the experiment progressed because the light reaction was able to occur. However, the dark cuvettes had stable levels of transmittance because light is necessary to excite electrons, which, in turn, reduces the DPIP.
Light transmittance can be more then 100 percent?
logically no because if it is a yes, then the light reaching the detector is greater than the light which was produced by the machine in the first place. But you may get transmittance greater than 100 because some parameters of your experiment may not be right.
How do you calculate ic50 for antioxidant activity?
Scavenging capacity (%) = 100 - [(absorbance of sample - absorbance of blank) × 100/absorbance of control] The tests were done in triplicate. The IC50 values were calculated by linear regression of plots, where the abscissa represents the concentration of the tested plant extracts and the ordinate the average percent of scavenging capacity. The concentration of sample required to scavenge 50% of DPPH (IC50) were determined.
What is the purpose of AP lab 4?
Exercise 4A: To separate and identify the various pigments (which absorb sunlight) in spinach leaves. Exercise 4B: To study the light-dependent reactions. We will be measuring the rate of DPIP reduced and the percent light transmittance.
How do you prepare the standard curve?
To prepare the standard curve you will need linear graph paper, semi-log graph paper and absorbance. You can define your standard curve by finding the absorption or percent plot on the Y axis.
