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Antimicrobial Agents and Chemotherapy, January 2010, p. 509-512, Vol. 54, No. 1
0066-4804/10/$12.00+0 doi:10.1128/AAC.00940-09
Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Method for Screening Compounds That Influence Virulence Gene Expression in Staphylococcus aureusAnita Nielsen,1 Kristian F. Nielsen,2 Dorte Frees,1 Thomas O. Larsen,2, and Hanne Ingmer1*Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, DK-1870 Frederiksberg, Denmark,1 Department of Systems Biology, Technical University of Denmark, DK-2800 Lyngby, Denmark2
Received 8 July 2009/ Returned for modification 3 August 2009/ Accepted 2 November 2009
We present a simple assay to examine effects of compounds on virulence gene expression in the human pathogen Staphylococcus aureus. The assay employs transcriptional reporter strains carrying lacZ fused to central virulence genes. Compounds affecting virulence gene expression and activity of the agrlocus are scored based on color change in the presence of a chromogenic β-galactosidase substrate. The assay can be used to screen for novel antivirulence compounds from many different sources, such as fungi, as demonstrated here.
* Corresponding author. Mailing address: Department of Veterinary Disease Biology, Stigboejlen 4, 1870 Frederiksberg C, Denmark. Phone: 45-3533-2773. Fax: 45-3528-2757. E-mail: hi@life.ku.dk
Published ahead of print on 16 November 2009.
Antimicrobial Agents and Chemotherapy, January 2010, p. 509-512, Vol. 54, No. 1
0066-4804/10/$12.00+0 doi:10.1128/AAC.00940-09
Copyright © 2010, American Society for Microbiology. All Rights Reserved.
http://aac.asm.org/cgi/content/abstract/54/1/509?maxtoshow=&hits=10&RESULTFORMAT=&fulltext=staphyloccocus&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT
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http://aac.asm.org/cgi/search?fulltext=staphyloccocus&submit=yes&x=0&y=0
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What are the conflict of that story of aged mother?
opo
What are the conflict of the story of aged mother?
opo
What is the conflict of the story The story of The Aged Mother?
The conflict in "The Story of the Aged Mother" revolves around the emperor's mandate to euthanize all elderly people in the kingdom. The aged mother and her son are forced to flee into the mountains to escape this decree, leading to a struggle between the son's loyalty to the emperor and his love for his mother. Ultimately, they find a way to fulfill the emperor's demand without causing harm to the aged mother.
What is denovement in the story of the aged mother?
In the story "The Aged Mother," the dénouement is when the decree to sacrifice all the elderly is lifted, the Emperor realizes the importance of his mother, and the mother and son are reunited. It serves as the resolution of the conflict and brings closure to the story.
What is the conflict of the story of the aged of mother?
The farmer is ordered to abandon his beloved mother to die because she is old and weak.
What is theme in the story of the aged mother?
the story of the aged mother
What is the theme in the story 'The Aged Mother'?
the story of the aged mother
What is the conflict man vs man in the story 'The Aged Mother'?
In the story "The Aged Mother," the conflict of man vs. man arises when the cruel decree of sacrificing elderly people is issued by the ruler, pitting the protagonist and his mother against the decree and the ruler's authority. The son's decision to defy the decree and save his mother's life encapsulates this conflict.
What is the exposition in the aged mother?
In the story "The Aged Mother," the exposition introduces the setting of a village where a cruel law dictates that all elderly people must be put to death. It also introduces the main characters, specifically the aged mother and her son, who come into conflict due to this unjust law. The exposition sets up the central conflict of the story as the aged mother faces the prospect of being abandoned.
What is the complication of the story of the aged mother?
The complication in the story of the aged mother involves the Emperor's decree to have all elderly people put to death, including the aged mother of the protagonist. This sets up the central conflict of the story as the son grapples with the difficult decision of whether to follow the decree or defy the Emperor to save his mother.
Who is the aged mother in the story of aged mother?
emperor
Who is the author of the story the aged mother?
The author of the story,''The story of the Aged Mother'' is Matsuo Basho
