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the counter stain is safranin 0.5%
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Why is it essential that the primary stain and the counterstain be of contrasting colors?
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
What does a positive result for spore stain indicate?
The spore was neutralized before stain removal insued.
Which step of a gram stain can be omitted without affecting determination of the reaction?
you can omit the last step - the safranin counterstain
What does a negative result for a spore stain indicate about the organism?
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
What is the meaning of stain for?
meaning of counterstain
Why is it essential that the primary stain and the counterstain be of contrasting colors?
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
What happen if you forget to add crystal violet to a stain?
You don't get a counterstain.
What does a positive result for spore stain indicate?
The spore was neutralized before stain removal insued.
In spore staining what would happen it you used safarin as the primary stain and malachite green as the counterstain?
The spore would appear to be red as the safranin is heat driven into the many layers of the spore, however, as Malachite green has a weak affinity and is water soluble, it will not likely bind to the spore wall or the cell wall. You might have traces of green on the slide if any, but it will be very little. Your vegetative cells will be pink as well.
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
Which step of a gram stain can be omitted without affecting determination of the reaction?
you can omit the last step - the safranin counterstain
What is the application of heat not required during the ap plication of the counterstain in acid fast stain?
17
Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
Can use carbolfuchsin as a counterstain?
The carbolfuchsin is a differential stain. It is an acid-fast stain which distinguishes organisms with waxy cell walls with the use of acid alcohol.
Why gram staining procedure cannot stain endospores?
Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
